马铃薯纺锤块茎类病毒在体内被绒毛烟草斑驳病毒颗粒包裹。
In vivo encapsidation of potato spindle tuber viroid by velvet tobacco mottle virus particles.
作者信息
Francki R I, Zaitlin M, Palukaitis P
机构信息
Department of Plant Pathology, Cornell University, Ithaca, New York 14853, USA.
出版信息
Virology. 1986 Dec;155(2):469-73. doi: 10.1016/0042-6822(86)90208-4.
When Nicotiana clevelandii was inoculated simultaneously with velvet tobacco mottle virus (VTMoV) and potato spindle tuber viroid (PSTV), replication of the viroid was greatly suppressed. However, both pathogens replicated if PSTV was inoculated several days before the VTMoV. Small amounts of PSTV were detected by dot-blot analysis of RNA in highly purified VTMoV isolated from coinfected plants indicating that the viroid had been encapsidated by the virus particles. Moreover, tomato plants which are immune to VTMoV became infected with PSTV when inoculated with VTMoV containing the viroid. Implications of these results are discussed in relation to questions regarding the spread and evolution of viroids and viroid-like satellite RNAs.
当用绒毛烟草斑驳病毒（VTMoV）和马铃薯纺锤块茎类病毒（PSTV）同时接种克利夫兰烟草时，类病毒的复制受到极大抑制。然而，如果在接种VTMoV前几天接种PSTV，两种病原体都会复制。通过对从共同感染植株中高度纯化的VTMoV中的RNA进行斑点印迹分析，检测到少量PSTV，这表明类病毒已被病毒粒子包裹。此外，对VTMoV免疫的番茄植株在接种含有该类病毒的VTMoV时会感染PSTV。结合有关类病毒和类病毒样卫星RNA的传播及进化问题，对这些结果的意义进行了讨论。
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