Erickson J W, Bancroft J B
Department of Plant Sciences, The University of Western Ontario, London, Ontario, Canada.
Virology. 1981 Jan 15;108(1):235-40. doi: 10.1016/0042-6822(81)90542-0.
The coat proteins of two rod-shaped plant viruses, papaya mosaic virus (PMV) and tobacco mosaic virus (TMV), have been tested for RNA melting activity. Under reconstitution conditions, PMV protein melts RNA in a noncooperative fashion. This activity is aspecific and is inhibited by low concentrations of NaCl as is virus reconstitution. TMV protein does not melt RNA either in the absence of NaCl or under reconstitution conditions at moderate ionic strength levels. The results suggest that elongated plant viruses have evolved at least two different assembly strategies in order to satisfy the requirement that the RNA within these viruses be in a melted configuration.
两种杆状植物病毒——木瓜花叶病毒（PMV）和烟草花叶病毒（TMV）的外壳蛋白已被检测其RNA解链活性。在重组条件下，PMV蛋白以非协同方式使RNA解链。这种活性是非特异性的，并且像病毒重组一样受到低浓度NaCl的抑制。在没有NaCl的情况下或在中等离子强度水平的重组条件下，TMV蛋白都不会使RNA解链。结果表明，细长的植物病毒已经进化出至少两种不同的组装策略，以满足这些病毒内的RNA处于解链构型的要求。
