Gingery R E, Nault L R, Bradfute O E
Agricultural Research, Science and Education Administration, U. S. Department of Agriculture, and Department of Entomology and Plant Pathology, Ohio Agricultural Research and Development Center, Wooster, Ohio 44691, USA.
Virology. 1981 Jul 15;112(1):99-108. doi: 10.1016/0042-6822(81)90616-4.
An unusual filamentous nucleoprotein about 3 nm in diameter was consistently associated with maize stripe-diseased maize. Antiserum to purified nucleoprotein neutralized the infectivity of extracts from maize stripe-diseased plants suggesting that the nucleoprotein was the maize stripe virus (MStpV). The rate-zonal sedimentation pattern of the nucleoprotein on sucrose gradients was polydisperse between 51 and 70 S. CsCl isopycnic centrifugation of combined nucleoprotein zones from sucrose gradients resulted in a single band of 1.28 g/ml. The nucleoprotein consisted of 5.2% RNA and a single capsid protein of molecular weight 32,700 daltons. Large quantities of a noncapsid protein of molecular weight 16,300 daltons were also found in MStpV-infected tissue. MStpV was transovarially transmitted by its vector, Peregrinus maidis. Other species susceptible to MStpV in addition to Zea mays were Rottboellia exaltata and Sorghum bicolor. The similarities between MStpV and rice stripe virus are discussed. We conclude that these viruses represent a new virus class.
一种直径约3纳米的异常丝状核蛋白始终与患玉米条纹病的玉米相关联。针对纯化核蛋白的抗血清可中和来自患玉米条纹病植株提取物的感染性，这表明该核蛋白就是玉米条纹病毒（MStpV）。核蛋白在蔗糖梯度上的速率区带沉降模式在51至70 S之间呈多分散性。对来自蔗糖梯度的合并核蛋白区带进行CsCl等密度离心，得到一条密度为1.28 g/ml的单一区带。该核蛋白由5.2%的RNA和一种分子量为32,700道尔顿的单一衣壳蛋白组成。在受MStpV感染的组织中还发现了大量分子量为16,300道尔顿的非衣壳蛋白。MStpV通过其介体玉米长蝽经卵传播。除玉米外，其他对MStpV敏感的物种还有高杆孔颖草和双色高粱。文中讨论了MStpV与水稻条纹病毒之间的相似性。我们得出结论，这些病毒代表了一个新的病毒类别。
