Sdoodee R, Teakle D S, Louie R
Prince of Songkla University, Hat Yai, 90110, Thailand.
University of Queensland, Brisbane Qld 4072, Australia.
Plant Dis. 1997 Feb;81(2):228. doi: 10.1094/PDIS.1997.81.2.228B.
Maize stripe tenuivirus (MStV) and its planthopper vector, Peregrinus maidis, occur in many tropical and subtropical regions worldwide (2). However, MStV has not been reported from the southeastern Asia continent. In January 1990, five of 200 Sorghum bicolor plants growing in Hat Yai, Thailand, showed symptoms typical of MStV, i.e., chlorotic spots, streaks, and wide bands on the younger leaves. Colonies of P. maidis also were present in leaf whorls of both symptomatic and asymptomatic sorghum plants. In December 1991, two sweet corn plants (Zea mays cv. Thai Super Sweet Composite 1 DMR) growing in the same area showed similar symptoms and were infested with P. maidis. When examined by phase-contrast microscopy, sap from chlorotic leaf tissue of one sweet corn plant had needle-shaped crystals typical of MStV infection (1). Sap from asymptomatic sweet corn plants had no crystals. In April 1996. maize cv. Chieng Mai 90 with MSt-like symptoms and also infested by P. maidis was collected from Hat Yai. Leaves were freeze dried, sent to Ohio, and tested by F(ab')2 enzyme-linked immunosorbent assay (ELISA). Positive control samples were fresh or freeze-dried leaves of sweet corn infected with MStV from Australia, Costa Rica, Malawi, Mauritius. and the U.S. Leaves of asymptomatic glasshouse-grown sweet corn seedlings were negative controls. In the ELISAs, the OD values at A of the Thailand sample with MStV symptoms was 1.00, compared with 0.33 to 1.52 for the positive control samples and 0.00 to 0.02 for fresh or freeze-dried negative controls. The microscopic and serological results indicate the occurrence of MStV in Thailand. References: (1) O. E. Bradfute and J. H. Tsai. Phytopathology 80:715, 1990. (2) R. E. Gingery et al. J. Gen. Virol. 64:1765, 1983.
玉米条纹纤细病毒（MStV）及其飞虱传播介体玉米蜡蝉在世界许多热带和亚热带地区均有发生（2）。然而，东南亚大陆尚未报道过MStV。1990年1月，泰国合艾种植的200株双色高粱中有5株表现出典型的MStV症状，即嫩叶上出现褪绿斑点、条纹和宽带。有症状和无症状高粱植株的叶腋中均有玉米蜡蝉群体。1991年12月，同一地区种植的两株甜玉米（玉米品种泰国超级甜复合1 DMR）表现出类似症状，并受到玉米蜡蝉侵害。通过相差显微镜检查，一株甜玉米褪绿叶片组织的汁液中有典型的MStV感染的针状晶体（1）。无症状甜玉米植株的汁液中没有晶体。1996年4月，从合艾采集到具有类似MSt症状且也受到玉米蜡蝉侵害的清迈90玉米品种。叶片经冷冻干燥后送往俄亥俄州，并通过F(ab')2酶联免疫吸附测定（ELISA）进行检测。阳性对照样品是来自澳大利亚、哥斯达黎加、马拉维、毛里求斯和美国的感染MStV的新鲜或冷冻干燥的甜玉米叶片。温室种植的无症状甜玉米幼苗叶片为阴性对照。在ELISA检测中，出现MStV症状的泰国样品在A 处的OD值为1.00，而阳性对照样品的OD值为0.33至1.52，新鲜或冷冻干燥阴性对照的OD值为0.00至0.02。显微镜和血清学结果表明泰国存在MStV。参考文献：（1）O. E. Bradfute和J. H. Tsai。植物病理学80:715，1990。（2）R. E. Gingery等人。普通病毒学杂志64:1765，1983。
