Moore N F, Reavy B, Pullin J S, Plus N
Natural Environment Research Council, Institute of Virology, 5 South Parks Road, Oxford OX1 3UB, United Kingdom.
Virology. 1981 Jul 30;112(2):411-6. doi: 10.1016/0042-6822(81)90288-9.
The Ouarzazate strain of Drosophila virus (DCV0) was grown in Drosophila melanogaster tissue culture cells, and [35S]methionine-labeled virions were found to contain a group of major structural proteins with a molecular weight of approximately 30,000 as well as several minor proteins of higher molecular weight and a protein of approximately 10,000 daltons. Using a range of pulses, chases and gel systems, examination of the intracellular proteins induced by DCV0 showed the presence of 17 polypeptides not found in uninfected cells. The synthesis of virus-induced polypeptides was extremely asymmetric with a rapid appearance of the major virus structural proteins and a much slower appearance of the lowest molecular weight structural protein (VP4). Processing of virus-induced proteins including the appearance of VP4 was demonstrated using pulse-chase after pulsing with [35S]methionine. While the highest molecular weight induced protein found in infected cells was 146,000, pretreatment of cells with iodoacetamide resulted in the appearance of a protein with a molecular weight of approximately 200,000. The evidence presented in this paper supports the inclusion of DCV0 in the Picornaviridae group.
果蝇病毒瓦尔扎扎特毒株（DCV0）在黑腹果蝇组织培养细胞中培养，发现用[35S]甲硫氨酸标记的病毒粒子含有一组分子量约为30,000的主要结构蛋白以及几种分子量较高的次要蛋白和一种约10,000道尔顿的蛋白。使用一系列脉冲、追踪和凝胶系统，对DCV0诱导的细胞内蛋白进行检测，结果显示存在17种未感染细胞中未发现的多肽。病毒诱导多肽的合成极其不对称，主要病毒结构蛋白迅速出现，而分子量最低的结构蛋白（VP4）出现得慢得多。在用[35S]甲硫氨酸脉冲后进行脉冲追踪，证明了病毒诱导蛋白的加工过程，包括VP4的出现。虽然在感染细胞中发现的分子量最高的诱导蛋白为146,000，但用碘乙酰胺预处理细胞会导致出现一种分子量约为200,000的蛋白。本文提供的证据支持将DCV0归入小核糖核酸病毒科。
