Leonard D A, Zaitlin M
Department of Plant Pathology, Cornell University, Ithaca, New York 14853, USA.
Virology. 1982 Mar;117(2):416-24. doi: 10.1016/0042-6822(82)90480-9.
An analysis has been made of the proteins synthesized in vivo and in vitro from two closely related tomato strains of tobacco mosaic virus, one of which is defective in cell-to-cell movement at a restrictive temperature [Nishiguchi et al. (1978). J. Gen. Virol. 39, 53-61]. The coat proteins of the two strains (L, and the temperature-sensitive LS-1) are identical as revealed by two-dimensional tryptic peptide maps prepared from proteins synthesized in vivo. Peptide maps of the 30,000, 130,000, and 165,000 Mr viral RNA in vitro translational products revealed a slight difference only in the 30,000 Mr polypeptides of the two strains, implicating this polypeptide as a factor mediating cell-to-cell movement of virus in the leaf.
对两种密切相关的烟草花叶病毒番茄株系在体内和体外合成的蛋白质进行了分析，其中一种在限制温度下细胞间移动存在缺陷[西口等人（1978年）。《普通病毒学杂志》39卷，53 - 61页]。从体内合成的蛋白质制备的二维胰蛋白酶肽图显示，这两种株系（L和温度敏感型LS - 1）的外壳蛋白是相同的。体外翻译产物中30000、130000和165000分子量病毒RNA的肽图仅显示两种株系在30000分子量多肽上有轻微差异，这表明该多肽是介导病毒在叶片中细胞间移动的一个因子。
