Department of Biochemistry Histochemistry and Cytochemistry, State University of Leiden, Wassenaarseweg 64, 2333 AL, Leiden, The Netherlands.
Plant Mol Biol. 1983 Jan;2(1):19-25. doi: 10.1007/BF00187572.
Immunofluorescence flow cytometry was used to study the distribution of viral antigen in protoplast populations. Protoplasts were isolated from healthy and alfalfa mosaic virus (AMV) infected tobacco leaves (designated in vivo infected). Furthermore isolated tobacco and cowpea protoplasts were infected in vitro with AMV. The FITC-conjugated antibodies could penetrate formaldehyde fixed protoplasts. The flow cytometric measurements were rapid and reproducible. Comparable immunofluorescence patterns were found for all infected samples (per sample 10(4) protoplasts were measured). Infectious virus could only be detected in in vivo infected tobacco protoplasts and in in vitro infected cowpea protoplasts.
免疫荧光流式细胞术用于研究病毒抗原在原生质体群体中的分布。原生质体从健康的和烟草花叶病毒（AMV）感染的烟草叶片（称为体内感染）中分离出来。此外，还从烟草和豇豆中分离原生质体，并用 AMV 体外感染。FITC 标记的抗体可以穿透甲醛固定的原生质体。流式细胞术测量快速且可重复。所有感染的样品都发现了类似的免疫荧光模式（每个样品测量 104 个原生质体）。只有在体内感染的烟草原生质体中和体外感染的豇豆原生质体中才能检测到感染性病毒。
