苜蓿银纹夜蛾核型多角体病毒DNA在细胞培养中连续传代后的变化

Alteration of Autographa californica nuclear polyhedrosis virus DNA upon serial passage in cell culture.

作者信息

Burand J P, Summers M D

机构信息

Department of Entomology Texas A&M University, College Station, Texas 77843, USA.

出版信息

Virology. 1982 May;119(1):223-9. doi: 10.1016/0042-6822(82)90082-4.

DOI:10.1016/0042-6822(82)90082-4

PMID:18635147

Abstract

Plaque-purified Autographa californica nuclear polyhedrosis virus, AcMNPV E2 isolate, was propagated for 30 generations by serial passage in TN-368 cells. After passage, the DNAs from 7 of 20 replaque-purified isolates were found to have additional EcoRI restriction enzyme fragments. These 7 isolates were of three types. The additional EcoRI fragments of each isolate hybridized to existing viral DNA. Restriction enzyme mapping with SacI and SmaI show that isolates of each type contain an insertion of repeated viral DNA in approximately the same region of the genome. The origin and location of this DNA is discussed.

摘要

通过在TN - 368细胞中连续传代，对空斑纯化的苜蓿银纹夜蛾核型多角体病毒（AcMNPV E2分离株）进行了30代繁殖。传代后，在20个再次空斑纯化的分离株中，有7个的DNA发现有额外的EcoRI限制性内切酶片段。这7个分离株分为三种类型。每个分离株的额外EcoRI片段与现有的病毒DNA杂交。用SacI和SmaI进行的限制性酶切图谱分析表明，每种类型的分离株在基因组的大致相同区域都含有重复病毒DNA的插入。本文讨论了该DNA的起源和位置。