斜纹夜蛾B型核多角体病毒基因组物理图谱

A physical map of Spodoptera littoralis B-type nuclear polyhedrosis virus genome.

作者信息

Croizier G, Boukhoudmi-Amiri K, Croizier L

机构信息

Station de Recherches de Pathologie Comparée, INRA-CNRS, Saint Christol-Lès-Alès, France.

出版信息

Arch Virol. 1989;104(1-2):145-51. doi: 10.1007/BF01313815.

DOI:10.1007/BF01313815

PMID:2647060

Abstract

A physical map for the genome of Spodoptera littoralis (Egyptian cottonworm) nuclear polyhedrosis virus of B-type (S1MNPV-B) [Cherry CL, Summers MD (1985) J Invertebr Pathol 46: 289-295] was prepared using the restriction endonucleases SmaI, SfiI, NotI, ApaI, KpnI, BstEII, PstI, SacI, and HindIII. The size of the genome was estimated to 136 kbp for the Morocco isolate (S1MNPV-M2) and to 135 kbp for the Lyon isolate (S1MNPV-L4). HindIII-K was used as the zero-point of the map because it hybridized to EcoRI-I and BamHI-F fragment of Autographa californica (AcMNPV) DNA, and the direction of transcription of the S1MNPV polyhedrin gene was chosen left to right. Hybridization of six AcMNPV cloned DNA fragments with S1MNPV-B genome shows that S1MNPV-B and AcMNPV genomic structures are not aligned.

摘要

使用限制性内切酶SmaI、SfiI、NotI、ApaI、KpnI、BstEII、PstI、SacI和HindIII构建了B型斜纹夜蛾核型多角体病毒（S1MNPV-B）[Cherry CL，Summers MD（1985）J Invertebr Pathol 46：289 - 295]的基因组物理图谱。摩洛哥分离株（S1MNPV-M2）的基因组大小估计为136千碱基对，里昂分离株（S1MNPV-L4）的基因组大小估计为135千碱基对。HindIII-K被用作图谱的零点，因为它与苜蓿银纹夜蛾核型多角体病毒（AcMNPV）DNA的EcoRI-I和BamHI-F片段杂交，并且S1MNPV多角体蛋白基因的转录方向选择为从左到右。六个AcMNPV克隆的DNA片段与S1MNPV-B基因组的杂交表明，S1MNPV-B和AcMNPV的基因组结构不一致。