Cheng Yiyun, Li Yiwen, Wu Qinglin, Zhang Jiahai, Xu Tongwen
Laboratory of Functional Membranes, Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, China.
Eur J Med Chem. 2009 May;44(5):2219-23. doi: 10.1016/j.ejmech.2008.05.031. Epub 2008 Jul 17.
The interactions of phenobarbital with different generations of amine-terminated poly(amidoamine) (PAMAM) dendrimers were investigated by using two dimensional-nuclear Overhauser effect spectroscopic (2D-NOESY) investigations. The NOESY spectra clearly showed that there were cross-peaks from NOE interactions between the protons of phenobarbital and the protons in interior cavities of generation 5 or generation 6 PAMAM dendrimers but none of such cross-peaks was found in the case of generation 3 or generation 4 dendrimers. The NOESY analysis, together with aqueous solubility data, suggested that higher generation dendrimers are more capable of encapsulating phenobarbital molecules into the interior cavities than lower generation dendrimers, and that lower generation dendrimers are much easier for the electrostatic attachment of phenobarbital molecules than higher ones at a fixed mass concentration.
通过二维核Overhauser效应光谱（2D - NOESY）研究，考察了苯巴比妥与不同代数的胺基封端聚（酰胺 - 胺）（PAMAM）树枝状大分子之间的相互作用。NOESY光谱清楚地表明，在苯巴比妥的质子与第5代或第6代PAMAM树枝状大分子内腔中的质子之间存在由NOE相互作用产生的交叉峰，但在第3代或第4代树枝状大分子的情况下未发现此类交叉峰。NOESY分析与水溶性数据表明，与较低代数的树枝状大分子相比，较高代数的树枝状大分子更能够将苯巴比妥分子包封到内腔中，并且在固定质量浓度下，较低代数的树枝状大分子比高代数的树枝状大分子更容易实现苯巴比妥分子的静电附着。
