Department of Cosmetology, University of Information Technology and Management in Rzeszów, Rzeszów, Poland.
Int J Nanomedicine. 2012;7:4819-28. doi: 10.2147/IJN.S34175. Epub 2012 Sep 6.
Bioconjugates of a polyamidoamine (PAMAM) G3 dendrimer and an aldehyde were synthesized as carriers for vitamins A and B₆, and the bioavailability of these vitamins for skin nutrition was investigated.
Nuclear magnetic resonance (NMR) and ultraviolet-visible methods were used to characterize the structure of the bioconjugates and for monitoring release of pyridoxal (Pyr) and pyridoxal phosphate (PLP) from these bioconjugates in vitro. A skin model permeation of bioconjugates was also studied in a Franz chamber.
A transdermal G3 PAMAM dendrimer was used to synthesize bioconjugates with trans-retinal (Ret), pyridoxal (Pyr), or PLP. These nanomolecules, containing up to four covalently linked Ret, Pyr, or PLP (G3(4Ret), G3(4Pyr), and G3(4PLP)), were able to permeate the skin, as demonstrated in vitro using a model skin membrane. PLP and Pyr bound to a macromolecular vehicle were active cofactors for glutamic pyruvic transaminase, as shown by ¹H NMR spectral monitoring of the progress of the L-alanine + α-ketoglutarate → glutamic acid + pyruvic acid reaction.
PAMAM-PLP, PAMAM-Pyr, and PAMAM-Ret bioconjugates are able to permeate the skin. PLP and Pyr are available as cofactors for glutamic pyruvic transaminase.
聚酰胺-胺（PAMAM）G3 树状大分子与醛的生物缀合物被合成作为维生素 A 和 B₆的载体，并研究了这些维生素对皮肤营养的生物利用度。
使用核磁共振（NMR）和紫外-可见方法来表征生物缀合物的结构，并监测这些生物缀合物在体外释放吡哆醛（Pyr）和吡哆醛磷酸（PLP）。还在 Franz 室中研究了生物缀合物的皮肤模型渗透。
使用透皮 G3 PAMAM 树状大分子合成了与视黄醛（Ret）、吡哆醛（Pyr）或 PLP 的生物缀合物。这些纳米分子，包含多达四个共价连接的 Ret、Pyr 或 PLP（G3(4Ret)、G3(4Pyr) 和 G3(4PLP)），能够渗透皮肤，如在体外使用模型皮肤膜所示。PLP 和 Pyr 与大分子载体结合是谷氨酸丙酮酸转氨酶的活性辅因子，如通过 ¹H NMR 光谱监测 L-丙氨酸+α-酮戊二酸→谷氨酸+丙酮酸反应的进展所示。
PAMAM-PLP、PAMAM-Pyr 和 PAMAM-Ret 生物缀合物能够渗透皮肤。PLP 和 Pyr 可作为谷氨酸丙酮酸转氨酶的辅因子。
