Department of Organic and Pharmaceutical Chemistry, Faculty of Pharmacy, Universidad Complutense, 28040, Spain.
Biotechnol Bioeng. 1997 Jul 20;55(2):252-60. doi: 10.1002/(SICI)1097-0290(19970720)55:2<252::AID-BIT2>3.0.CO;2-H.
Semipurified lipase of Candida rugosa (CRSL) was subjected to chemical modification, and the activities of the modified lipase, in hydrolysis and esterification reactions, were examined. The esterification reactions were carried out in the absence and presence of isooctane. When the enzyme was modified with polyethylene glycol (PEG), two methodologies were studied. The activation of PEG with p-NO(2)-phenylchloroformate gives better biocatalysts than those obtained with cyanuric chloride-PEG. The chemical modification with PEG increases the stability of pure lipases in isooctane at 50 degrees C (extreme conditions). The chemically modified enzymes are useful for biotransformations in organic solvents. In addition the nitration of tyrosines with tetranitromethane was also studied. (c) 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 55: 252-260, 1997.
假丝酵母脂肪酶（CRSL）经化学修饰后，对其在水解和酯化反应中的活性进行了研究。酯化反应在有无异辛烷的情况下进行。当用聚乙二醇（PEG）对酶进行修饰时，研究了两种方法。用对硝基苯氯甲酸酯活化 PEG 比用三聚氯氰-PEG 得到的生物催化剂更好。用 PEG 进行化学修饰提高了纯脂肪酶在 50 摄氏度异辛烷中的稳定性（极端条件）。化学修饰的酶可用于有机溶剂中的生物转化。此外，还研究了用四硝基甲烷对酪氨酸进行硝化。（c）1997 年 John Wiley & Sons，Inc.《生物技术与生物工程》55：252-260，1997 年。
