Matturri L, Campiglio G L, Lavezzi A M, Riberti C, Cavalca D, Azzolini A
Institute of Pathology, University of Milan, Italy.
Eur J Basic Appl Histochem. 1991;35(1):73-9.
One question which still remains unanswered concerning expansion of human skin is whether expansion simply stretches the skin or whether it induces a real neo-production of epithelium. To evaluate the proliferative activity of the human skin before and after expansion, we performed an autoradiographic study and a densitometric analysis on sections of skin biopsy from 11 patients. The results of the cellular kinetic study showed that expansion induces an increase in the epidermal proliferative activity. On the contrary, the densitometric analysis did not show a parallel increase of the proliferating cells after expansion. This discrepancy is discussed in terms of the modified morphology of the tissue. The proliferative activity of skin in the back region increases more than in that of the neck. Preliminary results have shown that the proliferative activity increases with time of expansion in days. The presence of a low percentage of cells with altered DNA content indicates, at least over the short term, the absence of dysplastic processes in expanded skins.
关于人类皮肤扩张仍未得到解答的一个问题是，扩张仅仅是拉伸皮肤，还是会诱导上皮细胞真正的新生。为了评估人类皮肤扩张前后的增殖活性，我们对11名患者的皮肤活检切片进行了放射自显影研究和密度测定分析。细胞动力学研究结果表明，扩张会诱导表皮增殖活性增加。相反，密度测定分析并未显示扩张后增殖细胞有平行增加。根据组织形态的改变对这种差异进行了讨论。背部皮肤的增殖活性比颈部增加得更多。初步结果表明，增殖活性随扩张天数的增加而增加。DNA含量改变的细胞比例较低，这表明至少在短期内，扩张后的皮肤不存在发育异常过程。
