Nitta Takahiro, Tanahashi Akihito, Obara Yu, Hirano Motohisa, Razumova Maria, Regnier Michael, Hess Henry
Department of Mathematical and Design Engineering, Gifu University, Japan.
Nano Lett. 2008 Aug;8(8):2305-9. doi: 10.1021/nl8010885. Epub 2008 Jul 18.
The design of nanoscale transport systems utilizing motor proteins as engines has advanced rapidly. Here, actin/myosin- and microtubule/kinesin-based molecular shuttles are compared with respect to their requirements for track designs. To this end, the trajectory persistence length of actin filaments gliding on myosin-coated surfaces has been experimentally determined to be equal to 8.8 +/- 2 microm. This measurement complements an earlier determination of the trajectory persistence length of microtubules gliding on kinesin-coated surfaces and enables a comparison of the accessible track designs for kinesin and myosin motor-powered systems. Despite the 200-fold smaller stiffness of actin filaments compared to that of microtubules, the dimensions of myosin tracks for actin filaments have to be quite similar to the dimensions of kinesin tracks for microtubules (radii larger than 200 nm and widths smaller than 0.9 microm compared to 600 nm and 19 microm). The difference in gliding speed is shown to require additional consideration in the design of track modules.
利用驱动蛋白作为引擎的纳米级运输系统设计发展迅速。在此，将基于肌动蛋白/肌球蛋白和微管/驱动蛋白的分子穿梭器在轨道设计要求方面进行了比较。为此，已通过实验确定在肌球蛋白包被表面上滑动的肌动蛋白丝的轨迹持续长度等于8.8±2微米。该测量补充了早期对在驱动蛋白包被表面上滑动的微管轨迹持续长度的测定，并能够比较驱动蛋白和肌球蛋白驱动系统可及的轨道设计。尽管肌动蛋白丝的刚度比微管小200倍，但肌动蛋白丝的肌球蛋白轨道尺寸必须与微管的驱动蛋白轨道尺寸非常相似（半径大于200纳米，宽度小于0.9微米，而微管的半径为600纳米，宽度为19微米）。结果表明，滑行速度的差异在轨道模块设计中需要额外考虑。
