El-Awar Nadim, Terasaki Paul I, Cai Junchao, Deng Chun-Tsan, Ozawa Miyuki, Nguyen Anh
One Lambda Inc., Canoga Park, CA, USA.
Clin Transpl. 2007:175-94.
The intent for this chapter was to summarize the HLA epitopes that have been defined by adsorption and elution of antibodies from single HLA antigens to date. Examples of reactions of mAb and eluted allosera with the class I, class II and MICA SA are also presented. We have identified 103 HLA class I epitopes, of which 40 were defined by mAbs and 63 by alloantibodies mostly eluted from rHLA class I single antigen cell lines. We identified 32 epitopes shared by A-locus antigens, 43 epitopes shared by B-locus antigens, 4 epitopes shared by C-locus antigens, 16 inter-locus epitopes shared by A-B loci antigens, 5 inter-locus epitopes shared by B-C loci antigens and 3 inter-locus epitopes shared by A-B-C-locus antigens. Sixty HLA-DR epitopes have been defined, most by one aa residue on the HLA DR beta chain. However, as is the case with Class I epitopes, some DR epitopes are defined by one or more alternative residue(s). Eighteen HLA-DQ epitopes have been identified on the HLA-DQB chain and on the HLA-DQA chain of HLA-DQ antigens. Most DQ epitopes were defined by one aa residue. However, almost half are characterized by several alternative combinations of aa residues. Only a few DQA epitopes have been identified. Seven MICA epitopes have been defined to date. All are defined by a single aa residue.
本章旨在总结迄今通过从单一HLA抗原吸附和洗脱抗体所确定的HLA表位。还展示了单克隆抗体（mAb）和洗脱的同种异体血清与I类、II类和MICA可溶性抗原（SA）反应的实例。我们已鉴定出103个HLA I类表位，其中40个由单克隆抗体确定，63个由主要从重组HLA I类单抗原细胞系洗脱的同种抗体确定。我们鉴定出A位点抗原共有的32个表位、B位点抗原共有的43个表位、C位点抗原共有的4个表位、A - B位点抗原共有的16个位点间表位、B - C位点抗原共有的5个位点间表位以及A - B - C位点抗原共有的3个位点间表位。已确定60个HLA - DR表位，大多数由HLA DRβ链上的一个氨基酸残基确定。然而，与I类表位情况相同，一些DR表位由一个或多个替代残基确定。已在HLA - DQ抗原的HLA - DQB链和HLA - DQA链上鉴定出18个HLA - DQ表位。大多数DQ表位由一个氨基酸残基确定。然而，几乎一半的表位具有几个氨基酸残基的替代组合特征。仅鉴定出少数几个DQA表位。迄今已确定7个MICA表位。所有表位均由单个氨基酸残基确定。
