烟草脉斑驳病毒RNA的无细胞翻译。II. 用针对柱状内含体、核内含体和辅助成分蛋白的抗血清对产物进行免疫沉淀。

Cell-free translation of tobacco vein mottling virus RNA. II. Immunoprecipitation of products by antisera to cylindrical inclusion, nuclear inclusion, and helper component proteins.

作者信息

Hellmann G M, Thornbury D W, Hiebert E, Shaw J G, Pirone T P, Rhoads R E

机构信息

Department of Biochemistry, University of Kentucky, Lexington, Kentucky 40536, USA.

出版信息

Virology. 1983 Jan 30;124(2):434-44. doi: 10.1016/0042-6822(83)90359-8.

DOI:10.1016/0042-6822(83)90359-8

PMID:18638869

Abstract

The genomic RNA of tobacco vein mottling virus (TVMV) was translated in a cell-free system derived from rabbit reticulocytes. Antisera against TVMV coat protein, TVMV cylindrical inclusions, the helper component required for aphid transmission of TVMV, and the 49- and 54-kd nuclear inclusion proteins of tobacco etch virus (TEV) were used to characterize the translational products. Each of the five antisera precipitated a distinctive pattern of polypeptides. Specificity of immunoprecipitation was shown by competing with the various proteins to which antisera were raised and by sequentially precipitating with two different antisera. These experiments showed that the five antisera define five different nonoverlapping regions of the TVMV genome.

摘要

烟草脉斑驳病毒（TVMV）的基因组RNA在源自兔网织红细胞的无细胞系统中进行翻译。使用针对TVMV外壳蛋白、TVMV柱状内含体、TVMV蚜虫传播所需辅助成分以及烟草蚀纹病毒（TEV）49-kd和54-kd核内含体蛋白的抗血清来鉴定翻译产物。五种抗血清中的每一种都沉淀出独特的多肽模式。通过与抗血清所针对的各种蛋白质竞争以及用两种不同抗血清依次沉淀来证明免疫沉淀的特异性。这些实验表明，这五种抗血清界定了TVMV基因组的五个不同且不重叠的区域。