马铃薯Y病毒基因组的克隆、定位及其体外表达
Cloning and mapping of the potato virus Y genome and its in vitro expression.
作者信息
Karchi M, Sela I, Edelbaum O, Ori A, Raccah B, Rosner A, Stram Y
机构信息
Virus Laboratory, Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot, Israel.
出版信息
Virus Genes. 1990 Sep;4(3):215-24. doi: 10.1007/BF00265631.
Full-length cDNA of genomic RNA of potato virus Y (PVY) was cloned in one piece into a lambda vector. The order of the EcoRI and SalI fragments of the inserted cDNA was determined. This is the first report of the cloning of a long, expressible, potyvirus genome. The availability of such a clone is a prerequisite for any further study of the molecular biology of this group of viruses, as they are expressed into a self-processed primary polyprotein.
马铃薯Y病毒（PVY）基因组RNA的全长cDNA被完整地克隆到一个λ载体中。确定了插入cDNA的EcoRI和SalI片段的顺序。这是关于一种长的、可表达的马铃薯Y病毒属病毒基因组克隆的首次报道。这样一个克隆的获得是对这类病毒分子生物学进行任何进一步研究的前提条件，因为它们会表达为一种自我加工的初级多聚蛋白。
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