Yamanaka Gaku, Ishii Chiako, Kawashima Hisashi, Oana Shingo, Miyajima Tasuku, Hoshika Akinori
Department of Pediatrics, Tokyo Medical University, Shinjuku, Tokyo, Japan.
Pediatr Neurol. 2008 Aug;39(2):80-4. doi: 10.1016/j.pediatrneurol.2008.04.006.
This study assessed the validity of cerebrospinal fluid oxidative status of pediatric patients with central nervous system diseases, using the Diacron-Reactive Oxygen Metabolites test (d-Roms; Diacron International s.r.l.; Grosseto, Italy). Eighty-seven pediatric patients (8 with bacterial meningitis, 18 with aseptic meningitis, 23 with febrile seizures, 6 with rotavirus gastroenteritis-induced convulsions, 16 with epilepsy, 2 with adrenoleukodystrophy, 2 with multiple sclerosis, and 12 control subjects) were enrolled. An analysis of the infection-associated group (bacterial meningitis, aseptic meningitis, febrile seizures, and rotavirus gastroenteritis-induced convulsions) indicated that cerebrospinal fluid Diacron-Reactive Oxygen Metabolite levels in the bacterial meningitis group were significantly higher than in other infection-associated groups. In the bacterial meningitis group, the cerebrospinal fluid Diacron-Reactive Oxygen Metabolite levels obtained after improvement were significantly decreased compared with pre-improvement values. In the noninfection-associated group (epilepsy, adrenoleukodystrophy, and multiple sclerosis), the cerebrospinal fluid Diacron-Reactive Oxygen Metabolite levels in symptomatic epilepsy patients were higher than in cryptogenic epilepsy patients and control subjects, but not significantly. Progressive patients with adrenoleukodystrophy or multiple sclerosis demonstrated high Diacron-Reactive Oxygen Metabolite levels compared with another early-stage adrenoleukodystrophy patient. Oxidative stress may be associated with the pathogenesis of various pediatric central nervous system diseases. Cerebrospinal fluid Diacron-Reactive Oxygen Metabolite levels may correlate with clinical status in these diseases.
本研究使用戴克隆活性氧代谢物检测法（d-Roms；戴克隆国际有限公司；意大利格罗塞托）评估了中枢神经系统疾病患儿脑脊液氧化状态的有效性。共纳入87名儿科患者（8例细菌性脑膜炎、18例无菌性脑膜炎、23例热性惊厥、6例轮状病毒性胃肠炎诱发惊厥、16例癫痫、2例肾上腺脑白质营养不良、2例多发性硬化症患者以及12名对照受试者）。对感染相关组（细菌性脑膜炎、无菌性脑膜炎、热性惊厥和轮状病毒性胃肠炎诱发惊厥）的分析表明，细菌性脑膜炎组脑脊液戴克隆活性氧代谢物水平显著高于其他感染相关组。在细菌性脑膜炎组中，病情改善后测得的脑脊液戴克隆活性氧代谢物水平与改善前的值相比显著降低。在非感染相关组（癫痫、肾上腺脑白质营养不良和多发性硬化症）中，症状性癫痫患者的脑脊液戴克隆活性氧代谢物水平高于隐源性癫痫患者和对照受试者，但差异不显著。与另一名早期肾上腺脑白质营养不良患者相比，进行性肾上腺脑白质营养不良或多发性硬化症患者的戴克隆活性氧代谢物水平较高。氧化应激可能与各种儿科中枢神经系统疾病的发病机制有关。脑脊液戴克隆活性氧代谢物水平可能与这些疾病的临床状态相关。
