Gray P L, Saggese M D, Phalen D N, Tizard I
The Schubot Exotic Bird Health Center, Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA.
Vet Immunol Immunopathol. 2008 Oct 15;125(3-4):216-24. doi: 10.1016/j.vetimm.2008.05.032. Epub 2008 Jun 12.
Creation of a reliable and easy to use serologic test would greatly improve ante mortem diagnosis of Mycobacterium avium subsp. avium and aid in the control of avian mycobacteriosis, particularly in captive birds. In order to determine whether serodiagnostics could be of value in testing ring-neck doves (Streptopelia risoria) for M. a. avium infection, Western blot analysis was used to assess the humoral response of ring-neck doves exposed to M. a. avium, and to evaluate whether an association could be made between the humoral response and necropsy findings, histopathology, culture, and PCR testing. Western blot results were examined for reactivity patterns associating humoral response with infection status, severity and type of lesions (diffuse vs. multifocal granulomatous inflammation) and phenotype (white vs. non-white). A sensitivity of 88.24% and a specificity of 100% were achieved utilizing Western blot analysis to detect M. a. avium infection in ring-neck doves, offering a negative predictive value of 93% and a positive predictive value of 100%. While Western blot analysis results did not reflect lesion severity, lesion type did partially correspond with the humoral response. The findings of the present study indicate that serologic testing can be used as a valuable ante mortem screening tool for identifying ring-neck doves infected with M. a. avium.
创建一种可靠且易于使用的血清学检测方法将极大地改善禽分枝杆菌亚种禽型分枝杆菌的生前诊断，并有助于控制禽分枝杆菌病，尤其是在圈养鸟类中。为了确定血清学诊断在检测环颈鸽（Streptopelia risoria）是否感染禽型分枝杆菌方面是否有价值，采用蛋白质印迹分析来评估暴露于禽型分枝杆菌的环颈鸽的体液免疫反应，并评估体液免疫反应与尸检结果、组织病理学、培养及聚合酶链反应检测之间是否存在关联。对蛋白质印迹结果进行检查，以寻找将体液免疫反应与感染状态、病变的严重程度和类型（弥漫性与多灶性肉芽肿性炎症）以及表型（白色与非白色）相关联的反应模式。利用蛋白质印迹分析检测环颈鸽中的禽型分枝杆菌感染，灵敏度达到88.24%，特异性达到100%，阴性预测值为93%，阳性预测值为100%。虽然蛋白质印迹分析结果未反映病变严重程度，但病变类型与体液免疫反应部分相符。本研究结果表明，血清学检测可作为一种有价值的生前筛查工具，用于识别感染禽型分枝杆菌的环颈鸽。
