Bolfion M, Salehi M, Ashrafi Helan J, Soleimani K, Keshavarz R, Aref Pajoohi R, Mohammad Taheri M, Tadayon K, Mosavari N
PPD Production Department, Razi Vaccine & Serum Research Institute, Karaj, Iran.
Iran J Microbiol. 2010 Dec;2(4):189-93.
Pigeons are extensively kept for homing and racing purposes in Iran. The main objective of this study was to investigate dissemination of M. avium subsp. avium (MAA) in pigeon aviaries in Tabriz, North-western Iran.
Postmortem pathologic specimens from thirty-nine out of 140 birds collected from private flocks (n=3), were subjected to bacterial culture out of which 3-4 mycobacterial isolates were recovered.
Applying a five-PCR diagnostic algorithm targeting short but definitive stretches of 16S rRNA and RV0577 genes, IS6110, IS901 and IS1245 genomic loci, proved all the isolates were MAA. They were either IS901+/IS1245+(n=22) or IS901+/IS1245- (n=12). When four healthy cattle sensitized against Mycobacterium bovis AN5 and Mycobacterium avium D4 were tuberculinated, the results confirmed the observed skin reactions against bovine tuberculin in animals sensitized with M. avium were large enough to complicate test interpretation.
We believe the extent of such epidemiological impact deserves further investigation if progress in control of bovine tuberculosis is intended.
在伊朗，鸽子被广泛饲养用于归巢和竞赛。本研究的主要目的是调查伊朗西北部大不里士鸽子饲养场中鸟型分枝杆菌鸟亚种（MAA）的传播情况。
从私人鸽群（n = 3）收集的140只鸟中的39只的死后病理标本进行细菌培养，从中分离出3 - 4株分枝杆菌。
应用针对16S rRNA和RV0577基因、IS6110、IS901和IS1245基因组位点的短而明确片段的五步PCR诊断算法，证明所有分离株均为MAA。它们要么是IS901 +/IS1245 +（n = 22），要么是IS901 +/IS1245 -（n = 12）。当对4头对牛分枝杆菌AN5和鸟分枝杆菌D4致敏的健康牛进行结核菌素试验时，结果证实，用鸟分枝杆菌致敏的动物中观察到的对牛结核菌素的皮肤反应足够大，足以使试验结果的解读复杂化。
我们认为，如果要在牛结核病控制方面取得进展，这种流行病学影响的程度值得进一步研究。
