Khan Alamgir, Williams Keith L, Soon Julie, Nevalainen Helena K M
Proteome Systems Ltd., 1/35-41 Waterloo Road, North Ryde, NSW 2113, Australia.
Mycol Res. 2008 Dec;112(Pt 12):1447-52. doi: 10.1016/j.mycres.2008.06.003. Epub 2008 Jul 2.
The soil-inhabiting, nematode-trapping fungus, Monacrosporium lysipagum, captures mobile stages of nematodes using specialized morphological structures, sticky knobs, that arise from mycelia. A study was conducted to separate the proteome of M. lysipagum mycelia containing knobs on two-dimensional (2D) gels resulting in a partial map of the proteome. The fungus was grown in a liquid soy peptone medium supplemented with the amino acids phenylalanine and valine to produce mycelia with knobs. Proteins extracted from the mycelia were separated by 2D gel electrophoresis and relatively high abundant proteins were identified by peptide mass fingerprinting (PMF). Out of the 250 proteins analysed by PMF, 51 (20%) were identified by cross-species matching due to unavailability of genomic information from M. lysipagum. This is the first published report on a proteomic analysis of a nematode-trapping fungus.
土壤栖息的捕食线虫真菌——莱氏单顶孢（Monacrosporium lysipagum），利用从菌丝体上产生的特殊形态结构——粘性瘤来捕获线虫的移动阶段。开展了一项研究，在二维（2D）凝胶上分离含有瘤的莱氏单顶孢菌丝体的蛋白质组，从而得到蛋白质组的部分图谱。该真菌在添加了苯丙氨酸和缬氨酸的液体大豆蛋白胨培养基中生长，以产生带有瘤的菌丝体。从菌丝体中提取的蛋白质通过二维凝胶电泳进行分离，并通过肽质量指纹图谱（PMF）鉴定相对高丰度的蛋白质。在通过PMF分析的250种蛋白质中，由于缺乏莱氏单顶孢的基因组信息，有51种（20%）通过跨物种匹配得以鉴定。这是关于捕食线虫真菌蛋白质组分析的首篇发表报告。
