Qin Lvgao, Liu Xiaoyong, Li Jun, Chen Huiqing, Yao Qin, Yang Zhe, Wang Lin, Chen Keping
Institute of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang 212013, Jiangsu Province, People's Republic of China.
Curr Microbiol. 2009 Jun;58(6):578-85. doi: 10.1007/s00284-009-9374-2. Epub 2009 Mar 14.
Nomuraea rileyi (N. rileyi) is the causative agent of the silkworm, Bombyx mori, green muscardine which can cause severe worldwide economical loss in sericulture. Little is known about N. rileyi at the protein level for this entomopathogenic parasite which belongs to the Ascomycota. Here, we employed proteomic-based approach to identify proteins of N. rileyi spores collected from the dead silkworm. In all, 252 proteins were separated by two-dimensional gel electrophoresis (2-DE), and were subjected to mass spectrometry (MS) analysis, 121 proteins have good MS signal, and 24 of them were identified due to unavailability of genomic information from N. rileyi. This data will be helpful in understanding the biochemistry of N. rileyi.
莱氏野村菌（N. rileyi）是家蚕绿僵病的病原体，这种疾病会在全球范围内给养蚕业造成严重的经济损失。对于这种属于子囊菌门的昆虫病原性寄生虫，在蛋白质水平上人们对莱氏野村菌了解甚少。在此，我们采用基于蛋白质组学的方法来鉴定从死亡家蚕中收集的莱氏野村菌孢子的蛋白质。总共通过二维凝胶电泳（2-DE）分离出了252种蛋白质，并对其进行了质谱（MS）分析，其中121种蛋白质具有良好的MS信号，由于缺乏莱氏野村菌的基因组信息，仅鉴定出了其中的24种。这些数据将有助于了解莱氏野村菌的生物化学特性。
