Bachi Angela, Bonaldi Tiziana
DIBIT, San Raffaele Scientific Institute, Milan, Italy.
J Proteomics. 2008 Aug 21;71(3):357-67. doi: 10.1016/j.jprot.2008.07.001. Epub 2008 Jul 9.
The definition of the role of each gene product in its cellular context is of outstanding importance in the post-genomics era. Recent technological innovations have driven research in proteomics from single protein characterization to global approaches, aiming to achieve a comprehensive qualitative and quantitative description of complex molecular mechanisms. In this review, we discuss the methodology of quantitative proteomics as it applies to the analysis of complex biological model systems. A special attention will be given to model systems that are suitable for functional genomic studies, where the potential of quantitative proteomics can be effectively demonstrated.
在后基因组时代，明确每个基因产物在其细胞环境中的作用定义至关重要。近期的技术创新推动了蛋白质组学研究从单一蛋白质表征转向全局方法，旨在对复杂的分子机制进行全面的定性和定量描述。在本综述中，我们讨论了定量蛋白质组学应用于复杂生物模型系统分析的方法。我们将特别关注适用于功能基因组学研究的模型系统，在这些系统中可以有效展示定量蛋白质组学的潜力。
