Chen Sixue, Harmon Alice C
Department of Botany and Genetics Institute, University of Florida, Gainesville, FL 32611-8526, USA.
Proteomics. 2006 Oct;6(20):5504-16. doi: 10.1002/pmic.200600143.
With the avalanche of genomic information and improvements in analytical technology, proteomics is becoming increasingly important for the study of many different aspects of plant functions. Since proteins serve as important components of major signaling and biochemical pathways, studies at protein levels are essential to reveal molecular mechanisms underlying plant growth, development, and interactions with the environment. The plant proteome is highly complex and dynamic. Although great strides need to be taken towards the ultimate goal of characterizing all the proteins in a proteome, current technologies have provided immense opportunities for high-throughput proteomic studies that have gone beyond simple protein identification to analyzing various functional aspects, such as quantification, PTM, subcellular localization, and protein-protein interactions. In this review of plant proteomics, advances in protein fractionation, separation, and MS will be outlined. Focus will be on recent development in functional analysis of plant proteins, which paves the way towards the comprehensive integration with transcriptomics, metabolomics, and other large scale "-omics" into systems biology.
随着基因组信息的大量涌现以及分析技术的进步，蛋白质组学对于研究植物功能的许多不同方面变得越来越重要。由于蛋白质是主要信号传导和生化途径的重要组成部分，因此在蛋白质水平上的研究对于揭示植物生长、发育以及与环境相互作用的分子机制至关重要。植物蛋白质组高度复杂且动态变化。尽管要实现对蛋白质组中所有蛋白质进行表征这一最终目标仍需迈出巨大步伐，但当前技术为高通量蛋白质组学研究提供了巨大机遇，这些研究已超越简单的蛋白质鉴定，深入到分析各种功能方面，如定量分析、翻译后修饰、亚细胞定位以及蛋白质 - 蛋白质相互作用。在这篇关于植物蛋白质组学的综述中，将概述蛋白质分级分离、分离以及质谱分析方面的进展。重点将放在植物蛋白质功能分析的最新进展上，这为与转录组学、代谢组学以及其他大规模“组学”全面整合到系统生物学中铺平了道路。
