Bagi C M, Miller S C
Division of Radiobiology, School of Medicine, University of Utah, Salt Lake City 84112.
Clin Orthop Relat Res. 1991 Aug(269):257-65.
The morphology, ultrastructure, tartrate-resistance acid phosphatase reactivity, and calcitonin responsiveness of osteoclasts induced at sites of demineralized bone matrix (DBM) implant-induced osteogenesis in rats were determined. Osteoclasts at these ectopic sites had a morphologic and ultrastructural appearance similar to osteoclasts normally found in skeletal tissues. When observed by scanning electron microscopy, resorption surfaces on the implants had well-defined resorption pits (Howship's lacunae), indicative of active bone resorption. The osteoclasts stained intensely for tartrate-resistance acid phosphatase, an enzyme that is specific for osteoclasts. In response to human calcitonin, hypocalcemia occurred and osteoclasts lost their ruffled borders, indicating that these cells are responsive to exogenous hormonal stimulation. The osteoclasts induced by subcutaneous implantation of DBM had morphologic and functional characteristics similar to osteoclasts normally found in skeletal tissues.
对大鼠脱矿骨基质（DBM）植入诱导成骨部位诱导产生的破骨细胞的形态、超微结构、抗酒石酸酸性磷酸酶反应性和降钙素反应性进行了测定。这些异位部位的破骨细胞在形态和超微结构上与骨骼组织中正常发现的破骨细胞相似。通过扫描电子显微镜观察时，植入物上的吸收表面有明确的吸收凹坑（豪希普陷窝），表明有活跃的骨吸收。破骨细胞对抵抗酒石酸的酸性磷酸酶染色强烈，这种酶是破骨细胞特有的。对人降钙素的反应是发生低钙血症，破骨细胞失去其皱襞缘，表明这些细胞对外源激素刺激有反应。皮下植入DBM诱导产生的破骨细胞具有与骨骼组织中正常发现的破骨细胞相似的形态和功能特征。
