Watanabe Y, Okada Y
Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Tokyo 113, Japan.
Virology. 1986 Feb;149(1):64-73. doi: 10.1016/0042-6822(86)90087-5.
A subcellular fraction which can synthesize viral RNA and subgenomic RNA in vitro was prepared from tobacco mosaic virus (TMV)-inoculated tobacco protoplasts. S(1)-Resistant fragment analysis with strand specific TMV cDNA showed that a large amount of plus-stranded and a small amount of minus-stranded, genome-size RNA was synthesized by this subcellular fraction. Plus-stranded subgenomic RNA of coat protein mRNA size was also synthesized. The time course of the appearance of viral RNA synthetic activity was consistent with that of the appearance of TMV infectivity in vivo.
从接种烟草花叶病毒（TMV）的烟草原生质体中制备了一种能够在体外合成病毒RNA和亚基因组RNA的亚细胞组分。利用链特异性TMV cDNA进行的S(1)抗性片段分析表明，该亚细胞组分合成了大量的正链和少量的负链基因组大小的RNA。还合成了外壳蛋白mRNA大小的正链亚基因组RNA。病毒RNA合成活性出现的时间进程与TMV在体内感染性出现的时间进程一致。
