Mommersteeg Paula M C, Vermetten Eric, Kavelaars Annemieke, Geuze Elbert, Heijnen Cobi J
Laboratory for Psychoneuroimmunology, University Medical Center Utrecht, KC03.068.0, Lundlaan 6, 3584EA Utrecht, The Netherlands.
Psychoneuroendocrinology. 2008 Sep;33(8):1041-50. doi: 10.1016/j.psyneuen.2008.05.007. Epub 2008 Jul 21.
Hostility is a risk factor for adverse health outcomes as diverse as cardiovascular disease and post-traumatic stress disorder (PTSD). Cytokines have been suggested to mediate this relationship. We investigated whether in healthy men a relation existed between hostility and T-cell mitogen-induced cytokines and chemokines. Male Dutch military personnel (n=304) were included before deployment. Eleven cytokines and chemokines were measured in supernatants of T-cell mitogen-stimulated whole blood cultures by multiplex immunoassay. Factor analysis was used to identify clusters of cytokines and chemokines. In a regression analysis hostility was related to the cytokine/chemokine clusters, and the potential risk factors age, BMI, smoking, drinking, previous deployment, early life trauma and depression. Explorative factor analysis showed four functional clusters; a pro-inflammatory factor (IL-2, TNFalpha, IFNgamma), an anti-inflammatory factor (IL-4, IL-5, IL-10), IL-6/chemokine factor (IL-6, MCP-1, RANTES, IP-10), and MIF. Hostility was significantly related to decreased IL-6/chemokine secretion and increased pro- and anti-inflammatory cytokines. There was an inverse relation between age and hostility scores. Early life trauma and depression were positively and independently related to hostility as well. This study represents a novel way of investigating the relation between cytokines and psychological characteristics. Cytokines/chemokines clustered into functional factors, which were related to hostility in healthy males. Moreover this relation appeared to be independent of reported depression and early trauma.
敌意是心血管疾病和创伤后应激障碍（PTSD）等多种不良健康结果的风险因素。细胞因子被认为介导了这种关系。我们调查了在健康男性中，敌意与T细胞丝裂原诱导的细胞因子和趋化因子之间是否存在关联。荷兰男性军事人员（n = 304）在部署前被纳入研究。通过多重免疫测定法测量T细胞丝裂原刺激的全血培养上清液中的11种细胞因子和趋化因子。使用因子分析来识别细胞因子和趋化因子的簇。在回归分析中，敌意与细胞因子/趋化因子簇以及潜在风险因素年龄、体重指数、吸烟、饮酒、先前部署、早年创伤和抑郁有关。探索性因子分析显示有四个功能簇；一个促炎因子（IL-2、TNFα、IFNγ），一个抗炎因子（IL-4、IL-5、IL-10），IL-6/趋化因子因子（IL-6、MCP-1、RANTES、IP-10）和MIF。敌意与IL-6/趋化因子分泌减少以及促炎和抗炎细胞因子增加显著相关。年龄与敌意得分呈负相关。早年创伤和抑郁也与敌意呈正相关且相互独立。本研究代表了一种研究细胞因子与心理特征之间关系的新方法。细胞因子/趋化因子聚集成功能因子，这些因子与健康男性的敌意有关。此外，这种关系似乎独立于报告的抑郁和早期创伤。
