de Loecker R, Goossens W, Bruneel P, van Duppen V, Penninckx F, Verwilghen R
Afdelingen Gastro-enterologische Heelkunde, Faculteit Geneeskunde, Katholieke Universiteit te Leuven, Belgium.
Cryobiology. 1991 Jun;28(3):237-45. doi: 10.1016/0011-2240(91)90028-m.
Cellular swelling of erythrocytes exposed to Me2SO during freezing and thawing may lead to hemolysis upon dilution of the cryoprotectant with pure electrolyte buffer. Excessive cell swelling is effectively avoided by exposing the RBC to the nonpenetrating sorbitol after thawing and before dilution. Due to the initial reduction in volume by sorbitol, cell swelling upon dilution may not cause hemolysis particularly with concentrations of 0.05 to 0.15 M of sorbitol in the diluting electrolyte buffer. Membrane damage incurred during freezing and thawing is particularly pronounced with the older red cell population, while the younger population membrane integrity can be preserved to an optimal degree.
在冻融过程中暴露于二甲基亚砜（Me2SO）的红细胞发生细胞肿胀，在用纯电解质缓冲液稀释冷冻保护剂时可能导致溶血。通过在解冻后和稀释前将红细胞暴露于非渗透性山梨醇，可以有效避免细胞过度肿胀。由于山梨醇使体积最初减小，稀释时的细胞肿胀可能不会引起溶血，尤其是在稀释电解质缓冲液中山梨醇浓度为0.05至0.15M时。在冻融过程中发生的膜损伤在较老的红细胞群体中尤为明显，而较年轻的红细胞群体的膜完整性可以得到最佳程度的保留。
