Zaug Arthur J, Podell Elaine R, Cech Thomas R
Howard Hughes Medical Institute and Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309-0215, USA.
Nat Struct Mol Biol. 2008 Aug;15(8):870-2. doi: 10.1038/nsmb.1462. Epub 2008 Jul 20.
Telomerase shows repeat-addition processivity (RAP): synthesis of multiple telomeric DNA repeats without primer dissociation. Leu14 mutants in the telomerase essential N-terminal domain of Tetrahymena thermophila telomerase reverse transcriptase retain full activity and anchor-site function but lose RAP, suggesting models for how this domain facilitates DNA translocation.
端粒酶表现出重复添加持续性（RAP）：即无需引物解离就能合成多个端粒DNA重复序列。嗜热四膜虫端粒酶逆转录酶的端粒酶必需N端结构域中的Leu14突变体保留了全部活性和锚定位点功能，但失去了RAP，这为该结构域如何促进DNA易位提供了模型。
