Cassai E, Manservigi R, Corallini A, Terni M
Intervirology. 1975;6(4-5):212-23. doi: 10.1159/000149476.
Polycaryocytogenic (P) and non-polycaryocytogenic, or aggregating (A), stable variants were selected from a herpes simplex virus type 1 (HSV-1) and from a herpes simplex virus type 2 (HSV 2) which had not been deliberately exposed to known mutagenic agents. The P variant of HSV-1 (FP) differed from the A variant (FA) in polypeptides and glycoprotein patterns, but no gross differences were evident between the two variants of HSV-2 (GP and GA). Each P variant proved more 'specific' than each A variant in immune neutralization tests. At high multiplicity, GP produced polycaryocytes but FP did not. Virulence tests in mice showed FP to be much more virulent than FA but GA to be more virulent than GP. Finally, A and P variants of each type could not be differentiated with respect to thermal resistance of virons, capacity to grow at high temperature, and buoyant density of DNA.
从单纯疱疹病毒1型（HSV-1）和单纯疱疹病毒2型（HSV-2）中筛选出多核细胞生成型（P）和非多核细胞生成型或聚集型（A）稳定变异体，这些病毒未曾特意暴露于已知诱变剂。HSV-1的P变异体（FP）在多肽和糖蛋白模式上与A变异体（FA）不同，但HSV-2的两个变异体（GP和GA）之间没有明显的总体差异。在免疫中和试验中，每个P变异体比每个A变异体表现出更强的“特异性”。在高感染复数时，GP能产生多核细胞而FP不能。小鼠毒力试验表明，FP的毒力比FA强得多，但GA的毒力比GP强。最后，就病毒粒子的热稳定性、在高温下生长的能力以及DNA的浮力密度而言，每种类型的A和P变异体无法区分。
