Nilheden E, Jeansson S, Vahlne A
Arch Virol. 1985;83(3-4):269-83. doi: 10.1007/BF01309922.
Clones of mouse neuroblastoma (Cl300) cells with increased resistance to herpes simplex virus (HSV) were obtained among survivors after prolonged exposure of partially HSV resistent Cl300 cells to successively increasing multiplicities of infection (MOI) of HSV. The increased restrictedness to HSV of these Cl300 R clones (Cl300 RI and Cl300 RII) as compared to the parental Cl300 cells was demonstrated by a tolerance to higher MOIs of HSV, judged by the appearance of cytopathic effects; by lower yields of progeny virus; and by higher activities of a non-interferon HSV inhibitor. Morphological appearance, cellular growth rate as well as HSV adsorptive capacity of the Cl300 R cells did not differ from that of Cl300 cells. Neither was virus penetration affected. These neuroblastoma Cl300 R cells, demonstrating an amplified resistance to HSV, might serve useful in studies on the regulation of virus replication in HSV latency establishment in neurons.
在部分对单纯疱疹病毒（HSV）有抗性的Cl300细胞长时间暴露于感染复数（MOI）不断增加的HSV后，从存活细胞中获得了对HSV抗性增强的小鼠神经母细胞瘤（Cl300）细胞克隆。与亲代Cl300细胞相比，这些Cl300 R克隆（Cl300 RI和Cl300 RII）对HSV的限制增加，这表现在对更高MOI的HSV具有耐受性，通过细胞病变效应的出现来判断；子代病毒产量较低；以及非干扰素HSV抑制剂的活性较高。Cl300 R细胞的形态外观、细胞生长速率以及HSV吸附能力与Cl300细胞没有差异。病毒穿透也未受影响。这些对HSV表现出增强抗性的神经母细胞瘤Cl300 R细胞，可能有助于研究HSV在神经元中建立潜伏期时病毒复制的调控。
