Grafton G, Bunce C M, Sheppard M C, Brown G, Baxter M A
Department of Medicine, University of Birmingham, Edgbaston, UK.
FEBS Lett. 1991 Jul 29;286(1-2):229-32. doi: 10.1016/0014-5793(91)80980-h.
When exposed to the phorbol ester TPA, HL60 cells undergo growth arrest and differentiate towards monocytes. During TPA-induced differentiation there was a 2.6-fold increase in the rate of inositol transport (Vmax), a 2.1-fold increase in intracellular inositol and a 1.5-fold increase in inositol lipid. An increase in the Vmax of inositol transport did not occur when the variant cell line HL60Ast3 was exposed to TPA, which has been shown in this cell line to induce growth arrest but not differentiation. This observation suggests that the change in inositol transport during HL60 monocyte differentiation is specifically associated with the process of cell differentiation as opposed to growth arrest.
当暴露于佛波酯TPA时，HL60细胞会发生生长停滞并向单核细胞分化。在TPA诱导的分化过程中，肌醇转运速率（Vmax）增加了2.6倍，细胞内肌醇增加了2.1倍，肌醇脂质增加了1.5倍。当变异细胞系HL60Ast3暴露于TPA时，肌醇转运的Vmax没有增加，在该细胞系中已表明TPA可诱导生长停滞但不能诱导分化。这一观察结果表明，HL60单核细胞分化过程中肌醇转运的变化与细胞分化过程特异性相关，而不是与生长停滞相关。
