Lord J M, Bunce C M, Duncan R J, Phillips I D, Shennan K I, Docherty K, Brown G
Department of Immunology, University of Birmingham Medical School, Edgbaston.
J Mol Endocrinol. 1988 Nov;1(3):197-201. doi: 10.1677/jme.0.0010197.
HL60 is a human promyeloid cell line capable of differentiating towards monocytes or granulocytes when treated with appropriate agents. Changes in insulin receptor number, affinity and mRNA levels were observed when HL60 cells were induced to differentiate with 12-O-tetradecanoyl-phorbol-13-acetate (TPA) or dimethylsulphoxide (DMSO). Total and high-affinity insulin receptor numbers decreased following treatment of HL60 cells with DMSO, whereas total insulin receptor number increased and high-affinity receptor number decreased in cells treated with TPA. Three distinct insulin receptor mRNA species of 9.1, 6.3 and 2.8 kb were identified in HL60 cells. The larger 9.1 and 6.3 kb species were increased in both TPA- and DMSO-treated HL60 cells, and the 2.8 kb mRNA was reduced in differentiated cells. Thus HL60 cells differentiated towards monocytes or granulocytes showed similar changes in the levels of individual insulin receptor mRNAs, but displayed contrasting alterations in low-affinity insulin binding. Three HL60 variant lines, which have different capacities to respond to inducers of monocyte and neutrophil differentiation, showed similar levels of total insulin receptors, but differed in their expression of high-affinity receptors. The data provide evidence for the existence of two distinct insulin receptors.
HL60是一种人类早幼粒细胞系，在用适当的试剂处理时能够向单核细胞或粒细胞分化。当HL60细胞用12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）或二甲基亚砜（DMSO）诱导分化时，观察到胰岛素受体数量、亲和力和mRNA水平的变化。用DMSO处理HL60细胞后，总胰岛素受体和高亲和力胰岛素受体数量减少，而用TPA处理的细胞中总胰岛素受体数量增加，高亲和力受体数量减少。在HL60细胞中鉴定出三种不同的胰岛素受体mRNA，大小分别为9.1、6.3和2.8 kb。在TPA和DMSO处理的HL60细胞中，较大的9.1和6.3 kb的mRNA种类均增加，而在分化细胞中2.8 kb的mRNA减少。因此，向单核细胞或粒细胞分化的HL60细胞在单个胰岛素受体mRNA水平上表现出相似的变化，但在低亲和力胰岛素结合方面表现出相反的改变。三种HL60变异株对单核细胞和中性粒细胞分化诱导剂的反应能力不同，它们的总胰岛素受体水平相似，但高亲和力受体的表达不同。这些数据为两种不同的胰岛素受体的存在提供了证据。
