Inokuchi J, Kumamoto Y, Jimbo M, Shimeno H, Nagamatsu A
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Fukuoka University, Japan.
FEBS Lett. 1991 Jul 29;286(1-2):39-43. doi: 10.1016/0014-5793(91)80935-v.
The effect of sphingosine (SPH) on the adhesive properties of Lewis lung carcinoma (3LL) cells was investigated using plastic precoated with the extracellular matrix proteins, laminin, fibronectin, or type IV collagen. Treatment of 3LL cells with SPH (0.5-10 microM) resulted in a dose-dependent decrease in the ability to bind to laminin and type IV collagen but had little or no effect on attachment to fibronectin. Phorbol 12-myristate 13-acetate (PMA) selectively enhanced attachment of 3LL cells to laminin and collagen. The inhibitory effect of SPH on attachment to both proteins was competitively antagonized by PMA. These results suggest that SPH acts as a negative effector for cell attachment to laminin and collagen, and that the cell attachment process to both proteins might be regulated in part by protein kinase C.
使用预先包被有细胞外基质蛋白（层粘连蛋白、纤连蛋白或IV型胶原）的塑料，研究了鞘氨醇（SPH）对Lewis肺癌（3LL）细胞黏附特性的影响。用SPH（0.5 - 10微摩尔）处理3LL细胞，导致其与层粘连蛋白和IV型胶原结合的能力呈剂量依赖性降低，但对其与纤连蛋白的附着影响很小或没有影响。佛波醇12 - 肉豆蔻酸酯13 - 乙酸酯（PMA）选择性增强了3LL细胞与层粘连蛋白和胶原的附着。PMA竞争性拮抗了SPH对两种蛋白附着的抑制作用。这些结果表明，SPH作为细胞附着于层粘连蛋白和胶原的负效应物，并且细胞对这两种蛋白的附着过程可能部分受蛋白激酶C调控。
