Kato S, Ben T L, De Luca L M
Differentiation Control Section, National Cancer Institute, Bethesda, Maryland 20892.
Exp Cell Res. 1988 Nov;179(1):31-41. doi: 10.1016/0014-4827(88)90345-x.
The effect of phorbol esters on the adhesive properties of NIH/3T3 mouse fibroblasts was investigated using plastic substrates precoated with the extracellular matrix proteins fibronectin, collagen, and laminin. Treatment with phorbol 12-myristate 13-acetate (PMA) enhanced NIH/3T3 cell attachment to laminin and type IV collagen substrates but had little or no effect on attachment to fibronectin and type I collagen substrates. The effect of PMA in enhancing cell attachment to laminin and type IV collagen substrates was dose dependent between 10(-9) and 10(-7) M. PMA was effective as early as 30 min; the effect reached a maximum at 2 h and decreased gradually. Phorbol 12, 13-dibenzoate and phorbol 12, 13-diacetate were effective but to a lesser extent and phorbol 12-myristate and phorbol 13-acetate showed little or no effect. These results suggest that PMA may enhance NIH/3T3 cell adhesion through effects on laminin and type IV collagen receptors. Retinoic acid, which itself requires at least 6 h to show an effect on attachment, did not have any effect on cell attachment in 2 h and, if anything, slightly inhibited PMA-enhanced cell attachment to laminin and type IV collagen substrates.
使用预先包被有细胞外基质蛋白纤连蛋白、胶原蛋白和层粘连蛋白的塑料底物，研究了佛波酯对NIH/3T3小鼠成纤维细胞黏附特性的影响。用佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）处理可增强NIH/3T3细胞对层粘连蛋白和IV型胶原底物的黏附，但对其对纤连蛋白和I型胶原底物的黏附影响很小或没有影响。PMA增强细胞对层粘连蛋白和IV型胶原底物黏附的作用在10^(-9)至10^(-7) M之间呈剂量依赖性。PMA早在30分钟时就有效；作用在2小时时达到最大值，然后逐渐下降。佛波醇12,13-二苯甲酸酯和佛波醇12,13-二乙酸酯有效，但程度较小，而佛波醇12-肉豆蔻酸酯和佛波醇13-乙酸酯几乎没有作用或没有作用。这些结果表明，PMA可能通过对层粘连蛋白和IV型胶原受体的作用来增强NIH/3T3细胞的黏附。视黄酸本身至少需要6小时才能显示出对黏附的作用，在2小时内对视黄酸对细胞黏附没有任何作用，而且如果有什么影响地话，它会轻微抑制PMA增强的细胞对层粘连蛋白和IV型胶原底物的黏附。
