Ueda Kumiko, Asai Yukiko, Yoshimura Yoshimizu, Iwakawa Seigo
Department of Pharmaceutics, Kobe Pharmaceutical University, 4-19-1 Motoyamakita-machi, Higashinada-ku, Kobe 658-8558, Japan.
J Pharm Pharmacol. 2008 Aug;60(8):1069-75. doi: 10.1211/jpp.60.8.0013.
The growth of human breast cancer-derived MCF-7 cells was affected by oil-in-water lipid emulsions prepared with fish oil (FO) rich in n-3 fatty acids (FAs) and egg-yolk phosphatides (EYP) (FO-emulsions), but not by lipid emulsions prepared with soybean oil (SO) and EYP (SO-emulsions). On the other hand, the growth of human hepatocarcinoma HepG2 cells was affected by neither SO-emulsions nor FO-emulsions. The growth inhibition of MCF-7 cells in the presence of FO-emulsions was not affected by trolox, but was inhibited by alpha-lipoic acid, and was even potentiated by ebselen, which works as an antioxidant as well as a lipoxygenase inhibitor. Since prostaglandin E(3), generated from n-3 FAs by cyclooxygenases, has a suppressive effect on tumour cell growth, and increases when lipoxygenases are inhibited, these findings suggest that lipid emulsions incorporating triglycerides of n-3 FAs might be effective in suppressing the growth of MCF-7 cells, possibly via oxidative stress and through eicosanoid production with anti-proliferating activity against cancer cells.
富含n-3脂肪酸（FAs）的鱼油（FO）和蛋黄磷脂（EYP）制备的水包油脂质乳剂（FO-乳剂）会影响人乳腺癌来源的MCF-7细胞的生长，但由大豆油（SO）和EYP制备的脂质乳剂（SO-乳剂）则不会。另一方面，人肝癌HepG2细胞的生长既不受SO-乳剂影响，也不受FO-乳剂影响。在FO-乳剂存在的情况下，MCF-7细胞的生长抑制不受生育三烯酚影响，但受α-硫辛酸抑制，甚至被兼具抗氧化剂和脂氧合酶抑制剂作用的依布硒仑增强。由于环氧化酶由n-3 FAs生成的前列腺素E(3)对肿瘤细胞生长有抑制作用，且在脂氧合酶被抑制时会增加，这些发现表明，包含n-3 FAs甘油三酯的脂质乳剂可能通过氧化应激以及产生具有抗癌细胞增殖活性的类花生酸来有效抑制MCF-7细胞的生长。
