Nutrition, Geneva University Hospital, Geneva, Switzerland.
Nutrition. 2013 Feb;29(2):450-6. doi: 10.1016/j.nut.2012.06.011. Epub 2012 Oct 22.
The anticancer action exerted by polyunsaturated fatty acid peroxidation may not be reproduced by commercially available lipid emulsions rich in vitamin E. Therefore, we evaluated the effects of fish oil (FO) emulsion containing α-tocopherol 0.19 g/L on human colorectal adenocarcinoma cells and tumors.
HT-29 cell growth, survival, apoptosis, and lipid peroxidation were analyzed after a 24-h incubation with FO 18 to 80 mg/L. Soybean oil (SO) emulsion was used as an isocaloric and isolipidic control. In vivo, nude mice bearing HT-29 tumors were sacrificed 7 d after an 11-d treatment with intravenous injections of FO or SO 0.2 g ∙ kg(-1) ∙ d(-1) FO or SO to evaluate tumor growth, necrosis, and lipid peroxidation.
The FO inhibited cell viability and clonogenicity in a dose-dependent manner, whereas SO showed no significant effect compared with untreated controls. Lipid peroxidation and cell apoptosis after treatment with FO 45 mg/L were increased 2.0-fold (P < 0.01) and 1.6-fold (P = 0.04), respectively. In vivo, FO treatment did not significantly affect tumor growth. However, immunohistochemical analyses of tumor tissue sections showed a decrease of 0.6-fold (P < 0.01) in the cell proliferation marker Ki-67 and an increase of 2.3-fold (P = 0.03) in the necrotic area, whereas malondialdehyde and total peroxides were increased by 1.9-fold (P = 0.09) and 7.0-fold (P < 0.01), respectively, in tumors of FO-treated compared with untreated mice.
These results suggest that FO but not SO has an antitumor effect that can be correlated with lipid peroxidation, despite its vitamin E content.
商业上可获得的富含维生素 E 的脂质乳剂所产生的多不饱和脂肪酸过氧化的抗癌作用可能无法重现。因此，我们评估了含有 0.19 g/Lα-生育酚的鱼油（FO）乳剂对人结直肠腺癌细胞和肿瘤的影响。
在 24 小时孵育期间，用 18 至 80 mg/L 的 FO 分析 HT-29 细胞的生长、存活、凋亡和脂质过氧化。使用大豆油（SO）乳液作为等热量和等脂乳液对照。在体内，在用 FO 或 SO（0.2 g·kg-1·d-1）静脉注射治疗 11 天后处死携带 HT-29 肿瘤的裸鼠，以评估肿瘤生长、坏死和脂质过氧化。
FO 以剂量依赖性方式抑制细胞活力和集落形成，而 SO 与未处理的对照相比没有显著影响。FO 45 mg/L 处理后，脂质过氧化和细胞凋亡分别增加了 2.0 倍（P < 0.01）和 1.6 倍（P = 0.04）。在体内，FO 治疗并未显著影响肿瘤生长。然而，肿瘤组织切片的免疫组织化学分析显示，细胞增殖标志物 Ki-67 减少了 0.6 倍（P < 0.01），坏死面积增加了 2.3 倍（P = 0.03），而丙二醛和总过氧化物分别增加了 1.9 倍（P = 0.09）和 7.0 倍（P < 0.01）。与未治疗的小鼠相比，FO 治疗的肿瘤中。
这些结果表明，FO 而不是 SO 具有抗肿瘤作用，尽管其维生素 E 含量较高，但可与脂质过氧化相关。
