Ward W Kenneth, Li Allen G, Siddiqui Yasmin, Federiuk Isaac F, Wang Xiao-Jing
Legacy Health System, 1225 NE 2nd Avenue, Portland, OR 97232, USA.
J Biomater Sci Polym Ed. 2008;19(8):1065-72. doi: 10.1163/156856208784909408.
The purpose of this study was to better understand whether interleukin-13 (IL-13) and connective tissue growth factor (CTGF) are highly expressed during foreign body encapsulation of subcutaneous devices. Mock biosensors were implanted into rats for three lengths of time (7-, 21- and 48-55 days) to address different stages of the foreign body response. Using quantitative real-time PCR and immunofluorescence, the expression of IL13, CTGF, collagen 1, decorin and fibronectin were measured in this tissue. IL-13, a product of Th2 cells, was highly expressed at all time points, with greatest expression at day 21. The IL-13 expression was paralleled by increased presence of T-cells at all time points. CTGF was also found to be more highly expressed in foreign body tissue than in controls. Collagen and decorin were highly expressed at the middle and later stages. Given the increased expression of IL-13 and CTGF in foreign body tissue, and their roles in other fibrotic disorders, these cytokines may well contribute to the formation of the foreign body capsule. Since the peak gene expression of IL-13 occurred later than the previously-reported TGFbeta expression peak, IL-13 is probably not the major stimulus to TGFbeta expression during foreign body encapsulation and may contribute to fibrosis independently.
本研究的目的是更好地了解白细胞介素-13（IL-13）和结缔组织生长因子（CTGF）在皮下装置的异物包裹过程中是否高表达。将模拟生物传感器植入大鼠体内三个不同时长（7天、21天以及48 - 55天），以研究异物反应的不同阶段。运用定量实时聚合酶链反应和免疫荧光技术，检测该组织中IL13、CTGF、胶原蛋白1、核心蛋白聚糖和纤连蛋白的表达。IL-13作为Th2细胞的产物，在所有时间点均高表达，在第21天时表达量最高。在所有时间点，IL-13的表达与T细胞数量的增加呈平行关系。还发现CTGF在异物组织中的表达也高于对照组。胶原蛋白和核心蛋白聚糖在中期和后期高表达。鉴于异物组织中IL-13和CTGF表达增加，以及它们在其他纤维化疾病中的作用，这些细胞因子很可能促成了异物包膜的形成。由于IL-13的基因表达峰值出现时间晚于先前报道的转化生长因子β（TGFβ）表达峰值，IL-13可能不是异物包裹过程中TGFβ表达的主要刺激因素，可能独立促成纤维化。
