Volkman L E, Goldsmith P A, Hess R T
Department of Entomology and Parasitology, University of California, Berkeley, California 94720, USA.
Virology. 1987 Jan;156(1):32-9. doi: 10.1016/0042-6822(87)90433-8.
Autographa californica nuclear polyhedrosis virus (AcNPV) is a large, double-stranded DNA virus that infects lepidopteran insects. In the course of infection two different forms of the virus are produced; one that serves to spread the infection from insect to insect and another that spreads the infection within the insect from cell to cell. The latter form matures by budding from the plasma membrane of infected cells. We have found that cytochalasins B and D prevent the production of infectious budded AcNPV, not by inhibiting budding but by inhibiting the synthesis of complete virions. In the presence of cytochalasins B and D, particles lacking nucleocapsids bud from the plasma membrane of AcNPV infected cells, implicating microfilaments in the synthesis, transport and/or assembly of critical virus components.
苜蓿银纹夜蛾核型多角体病毒（AcNPV）是一种大型双链DNA病毒，可感染鳞翅目昆虫。在感染过程中会产生两种不同形式的病毒；一种用于在昆虫之间传播感染，另一种用于在昆虫体内从一个细胞传播到另一个细胞。后一种形式通过从受感染细胞的质膜出芽而成熟。我们发现细胞松弛素B和D可阻止感染性出芽AcNPV的产生，不是通过抑制出芽，而是通过抑制完整病毒粒子的合成。在细胞松弛素B和D存在的情况下，缺乏核衣壳的颗粒从AcNPV感染细胞的质膜出芽，这表明微丝参与了关键病毒成分的合成、运输和/或组装。
