Department of Microbiology and Immunology, Queen's University, K7L 3N6, Kingston, ON, Canada.
Cytotechnology. 1996 Jan;20(1-3):95-110. doi: 10.1007/BF00350391.
In vitro studies have contributed greatly to an understanding of viral cytopathology, molecular biology, and pathogenesis. A model of the role of baculoviruses in a host-parasite relationship is developing which reveals the virus as gaining control of many aspects of host cell biology including control of the cell replication machinery (apoptotic response, macromolecular synthesis), the cytoskeletal structure, the nuclear membrane and intranuclear architecture. Baculovirus replication is a collection of independent but inter-related processes which work within the framework of the host cell, with the in vivo goal of maximizing production of progeny virions. Further molecular dissection of baculovirus replication should yield insight into the processes and principles of viral and host regulatory systems, perhaps facilitating development of new generations of high efficiency sub-viral expression vector systems and the development of genetically improved strains of virus safe for field use in ecologically based pest management strategies.
体外研究极大地促进了对病毒细胞病理学、分子生物学和发病机制的理解。正在形成杆状病毒在宿主-寄生虫关系中的作用模型，该模型揭示了病毒对宿主细胞生物学的许多方面的控制，包括对细胞复制机制（凋亡反应、大分子合成）、细胞骨架结构、核膜和核内结构的控制。杆状病毒的复制是一组独立但相互关联的过程，这些过程在宿主细胞的框架内运作，其体内目标是最大限度地产生后代病毒粒子。对杆状病毒复制的进一步分子剖析应该能够深入了解病毒和宿主调节系统的过程和原理，也许有助于开发新一代高效亚病毒表达载体系统，并开发用于生态为基础的害虫管理策略中田间使用的安全的遗传改良病毒株。
