Carstens E B, Tjia S T, Doerfler W
Institute of Genetics, University of Cologne, Cologne, Germany.
Virology. 1980 Feb;101(1):311-4. doi: 10.1016/0042-6822(80)90511-5.
Cells from the lepidopteran Spodoptera frugiperda can be successfully transfected in culture with the DNA from Autographa californica nuclear polyhedrosis virus (AcNPV). The calcium chloride precipitation procedure has been used in conjunction with dimethyl sulfoxide treatment of the transfected cells. The highest specific infectivity observed was 6.1 x 10(4) PFU/mug of AcNPV DNA. As judged from a comparison of the restriction patterns of viral DNA preparations, the virus produced in transfection experiments was identical to the virions from which the DNA for transfection was prepared. The transfection procedure described will be useful for the genetic analysis of AcNPV DNA.
来自鳞翅目昆虫草地贪夜蛾的细胞能够在培养中成功地用苜蓿银纹夜蛾核型多角体病毒（AcNPV）的DNA进行转染。氯化钙沉淀法已与转染细胞的二甲基亚砜处理结合使用。观察到的最高比感染性为6.1×10⁴ PFU/μg AcNPV DNA。从病毒DNA制剂的限制性图谱比较判断，转染实验中产生的病毒与用于转染的DNA所来自的病毒粒子相同。所描述的转染程序将有助于对AcNPV DNA进行遗传分析。
