花椰菜花叶病毒复制中间体被包装到病毒样颗粒中。

Cauliflower mosaic virus replication intermediates are encapsidated into virion-like particles.

作者信息

Marsh L E, Guilfoyle T J

机构信息

Department of Botany, University of Minnesota, St. Paul, Minnesota 55108, USA.

出版信息

Virology. 1987 Nov;161(1):129-37. doi: 10.1016/0042-6822(87)90178-4.

DOI:10.1016/0042-6822(87)90178-4

PMID:18644579

Abstract

We have identified putative cauliflower mosaic virus (CaMV) replication intermediates of first (minus)-strand DNA synthesis which are associated with particles having properties similar to virions. The virion-like particles containing the putative replication intermediates sediment with CaMV virions on sucrose density gradients (L. Marsh, A. Kuzj, and T. Guilfoyle (1985) Virology 143, 212-223), band close to virions on CsCl density gradients, and are precipitable with antibody raised against the CaMV 42-kDa coat protein. The structures of the presumptive intermediates of DNA synthesis are compatible with a reverse transcription model for CaMV. Most or all of the observed minus-strand replication complexes appear to exist as RNA/DNA hybrids. Following RNase A treatment at low salt concentrations, the putative replication intermediates show characteristics of single-stranded DNA in selectively blotting and hybridizing to minus-strand-specific probes without prior denaturation of the blots.

摘要

我们已经鉴定出了与具有类似于病毒粒子特性的颗粒相关的花椰菜花叶病毒（CaMV）第一（负）链DNA合成的假定复制中间体。含有假定复制中间体的病毒样颗粒在蔗糖密度梯度上与CaMV病毒粒子一起沉降（L. Marsh、A. Kuzj和T. Guilfoyle（1985年），《病毒学》143卷，212 - 223页），在CsCl密度梯度上与病毒粒子靠近的位置形成条带，并且能用针对CaMV 42 kDa外壳蛋白产生的抗体沉淀。DNA合成假定中间体的结构与CaMV的逆转录模型相符。大多数或所有观察到的负链复制复合体似乎以RNA/DNA杂交体的形式存在。在低盐浓度下用核糖核酸酶A处理后，假定的复制中间体在选择性印迹和与负链特异性探针杂交时，在印迹未预先变性情况下表现出单链DNA的特征。