Hull R, Covey S N
Nucleic Acids Res. 1983 Mar 25;11(6):1881-95. doi: 10.1093/nar/11.6.1881.
Several different forms of cauliflower mosaic virus (CaMV) DNA were detected in nucleic acid preparations from CaMV-infected turnip leaves. As well as supercoiled and open-circular molecules, various linear DNA structures were identified. The relative amounts of these DNA forms varied in plants infected with different CaMV isolates. Restriction enzyme mapping and one- and two-dimensional gel electrophoresis revealed the presence of linear molecules apparently formed by breaks in the second strand at each of the three discontinuities. Two major linear DNA forms are double-stranded over part of their length and appear to have single-stranded extensions of the -strand of variable length. Since these DNA forms are not produced during extraction and probably exist as unencapsidated or partially encapsidated molecules, they may represent intermediates either in DNA replication or in virion assembly.
在感染花椰菜花叶病毒（CaMV）的芜菁叶片核酸制剂中检测到了几种不同形式的CaMV DNA。除了超螺旋分子和开环分子外，还鉴定出了各种线性DNA结构。这些DNA形式的相对含量在感染不同CaMV分离株的植物中有所不同。限制性内切酶图谱分析以及一维和二维凝胶电泳显示，存在明显由三条不连续处第二条链断裂形成的线性分子。两种主要的线性DNA形式在其部分长度上是双链的，并且似乎具有长度可变的负链单链延伸。由于这些DNA形式在提取过程中不会产生，并且可能以未包装或部分包装的分子形式存在，它们可能代表DNA复制或病毒粒子组装过程中的中间体。
