Hu Jian-Hong, Li Qing-Wang, Li Gang, Jiang Zhong-Liang, Bu Shu-hai, Yang Hai, Wang Li-Qiang
Northwest A & F University, Yangling, ShaanXi, China.
Anim Reprod Sci. 2009 May;112(1-2):107-18. doi: 10.1016/j.anireprosci.2008.04.009. Epub 2008 Apr 24.
In order to improve boar sperm quality during frozen-thawed process, the influence of the presence of trehalose on success of cryopreservation of boar sperm were investigated. We evaluated freeze-thawing tolerance of boar spermatozoa in a base cooling extender with the addition of different trehalose concentrations (0, 25, 50, 100 and 200mmol/l), and tried to determine the optimum concentration of trehalose. We chose sperm motility, acrosome integrity, membrane integrity and cryocapacitation as parameters to evaluate cryopreservation capacity of boar spermatozoa. We obtained the best results for 100mmol/l trehalose-supplemented extenders, with values of 49.89% for motility, 66.52% for acrosome integrity and 44.61% for membrane integrity, while freeze-thawing tolerance was diminished significantly for 200mmol/l of trehalose. Before and after capacitation, the CTC score for semen diluted by extender containing 100mmol/l trehalose was 3.68% and 43.82%, respectively. In conclusion, trehalose could confer a greater cryoprotective capacity to boar spermatozoa. Trehalose-supplementation with 100mmol/l concentration in basic extender could significantly improve sperm motility, membrane integrity and acrosome integrity parameters, and reduce boar spermatozoa cryocapacitation during the cryopreservation process.
为了提高公猪精子在冻融过程中的质量，研究了海藻糖的存在对公猪精子冷冻保存成功率的影响。我们在基础冷冻稀释液中添加不同浓度的海藻糖（0、25、50、100和200mmol/L），评估公猪精子的冻融耐受性，并试图确定海藻糖的最佳浓度。我们选择精子活力、顶体完整性、膜完整性和冷冻获能作为参数来评估公猪精子的冷冻保存能力。在添加100mmol/L海藻糖的稀释液中我们获得了最佳结果，活力值为49.89%，顶体完整性为66.52%，膜完整性为44.61%，而在200mmol/L海藻糖时冻融耐受性显著降低。在获能前后，用含100mmol/L海藻糖的稀释液稀释的精液的CTC评分分别为3.68%和43.82%。总之，海藻糖可以赋予公猪精子更大的冷冻保护能力。在基础稀释液中添加100mmol/L浓度的海藻糖可以显著改善精子活力、膜完整性和顶体完整性参数，并降低公猪精子在冷冻保存过程中的冷冻获能。
