海藻糖添加对公猪精子质量的冷冻保护作用。
The cryoprotective effect of trehalose supplementation on boar spermatozoa quality.
作者信息
Hu J-H, Li Q-W, Jiang Z-L, Yang H, Zhang S-S, Zhao H-W
机构信息
College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi, China.
出版信息
Reprod Domest Anim. 2009 Aug;44(4):571-5. doi: 10.1111/j.1439-0531.2007.00975.x. Epub 2009 May 19.
In order to improve boar sperm quality during frozen-thawed process, the influence of the presence of trehalose on success of cryopreservation of boar sperm were investigated. We evaluated freeze-thawing tolerance of boar spermatozoa in a base cooling extender with the addition of different trehalose concentrations (0, 25, 50, 100 and 200 mm), and try to determine the optimum concentration of trehalose. We chose sperm motility, mitochondrial activity, acrosome integrity and membrane integrity as parameters to evaluate cryopreservation capacity of boar spermatozoa. We obtained the best results for 100 mm trehalose-supplemented extenders, with values of 49.89% for motility, 44.69% for mitochondrial activity, 66.52% for acrosome integrity and 44.61% for membrane integrity, while freeze-thawing tolerance diminished significantly for 200 . The synergic effect of trehalose and glycerol resulted in better cryosurvival of boar spermatozoa than that of a single cryoprotectant. In conclusion, when trehalose-supplementation was added up to 100 mm, trehalose confers a greater cryoprotective capacity to the extender, and the sperm motility, mitochondrial activity, membrane integrity and acrosome integrity parameters were significantly improved during frozen-thawed process.
为了提高公猪精子在冻融过程中的质量，研究了海藻糖的存在对公猪精子冷冻保存成功率的影响。我们评估了在添加不同浓度海藻糖（0、25、50、100和200 mM）的基础冷却稀释液中公猪精子的冻融耐受性，并试图确定海藻糖的最佳浓度。我们选择精子活力、线粒体活性、顶体完整性和膜完整性作为评估公猪精子冷冻保存能力的参数。在添加100 mM海藻糖的稀释液中我们获得了最佳结果，活力值为49.89%，线粒体活性为44.69%，顶体完整性为66.52%，膜完整性为44.61%，而在200 mM时冻融耐受性显著降低。海藻糖和甘油的协同作用导致公猪精子的冷冻存活率高于单一冷冻保护剂。总之，当添加的海藻糖浓度达到100 mM时，海藻糖赋予稀释液更大的冷冻保护能力，并且在冻融过程中精子活力、线粒体活性、膜完整性和顶体完整性参数得到显著改善。
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