Musgrave A, Häder D P
Department of Molecular Cell Biology, University of Amsterdam, The Netherlands.
Gen Physiol Biophys. 1991 Apr;10(2):189-99.
The phototactic behavior of Chlamydomonas eugametos gametes and vis-à-vis pairs was quantitated using a fully automated, computer-controlled microvideo image analysis system. Two different mt- (mating type minus) and one mt+ (mating type plus) strain, together with the two combinations of pairs were studied. One mt- strain of dark-adapted gametes was non-phototactic while the others were positively phototactic at all effective intensities of white light. The mt+ strain exhibited one of the strongest positive responses that has so far been reported in algae (r-values greater than 0.7). After sexual fusion, the mt+ cell powers the swimming vis-à-vis pair. Its phototactic behavior reversed on fusion, with the pairs swimming away from all effective light intensities, irrespective of whether its partner was formerly phototactic or not. However, when adapted to the dark for an hour or more, vis-à-vis pairs swam positively to the light. The ecological consequence could be that pairs settle and develop into zygotes under intermediate light intensities or at light-dark interfaces.
使用全自动、计算机控制的显微视频图像分析系统对衣藻（Chlamydomonas eugametos）配子及相对配对的趋光行为进行了定量分析。研究了两种不同的mt-（负交配型）和一种mt+（正交配型）菌株，以及两种配对组合。一种暗适应的mt-菌株的配子无趋光性，而其他菌株在所有有效白光强度下均表现出正趋光性。mt+菌株表现出迄今为止在藻类中报道的最强正反应之一（r值大于0.7）。有性融合后，mt+细胞驱动相对配对游动。其趋光行为在融合后发生逆转，配对会游离所有有效光强度，无论其伴侣之前是否具有趋光性。然而，当在黑暗中适应一小时或更长时间后，相对配对会向光正向游动。其生态结果可能是配对在中等光强度或明暗界面处沉降并发育成合子。
