De Rosa L, Montuoro A, Pandolfi A, Lanti T, Pescador L, Morara R, De Laurenzi A
Divisione di Ematologia, Ospedale San Camillo, Roma, Italy.
Haematologica. 1991 Mar;76 Suppl 1:37-40.
In this report we describe the use of the immunomagnetic depletion of tumor cells from harvested bone marrow in two patients with acute lymphoblastic leukemia. The immunomagnetic procedure of purging involved one incubation of the marrow cells with a mixture of 4 monoclonal antibodies which bind to antigens of CD10 (ALB2), CD19 (HD37), CD20 (B1) and CD22 (HD39). Two incubations with magnetic beads (Dynabeads M-450) covered with antimouse antibodies followed by magnetic separation were performed. The bead/target B cell ratio was 50:1. After purging the recovery of mononuclear cells was 56% and 40%, while the recovery of CFU-GM was 45% and 38% respectively. Both patients engrafted rapidly without serious complications. One patient relapsed 4 months after transplant, the other remains in complete remission after 5 months. Our results confirm that the use of immunomagnetic beads is a simple, safe and reproducible technique to remove tumor cells before ABMT in patients with B malignancies using a broad mixture of MoAbs. However only a randomized trial using autologous marrow purged or not will clarify the effective clinical value of the procedure.
在本报告中，我们描述了在两名急性淋巴细胞白血病患者中使用免疫磁珠从采集的骨髓中清除肿瘤细胞的情况。清除肿瘤细胞的免疫磁珠法包括将骨髓细胞与4种单克隆抗体的混合物孵育一次，这4种单克隆抗体分别针对CD10（ALB2）、CD19（HD37）、CD20（B1）和CD22（HD39）的抗原。之后进行两次与包被抗小鼠抗体的磁珠（Dynabeads M - 450）的孵育，随后进行磁分离。磁珠与靶B细胞的比例为50:1。清除肿瘤细胞后，单核细胞的回收率分别为56%和40%，而CFU - GM的回收率分别为45%和38%。两名患者均迅速植入，未出现严重并发症。一名患者在移植后4个月复发，另一名患者在5个月后仍处于完全缓解状态。我们的结果证实，使用免疫磁珠是一种简单、安全且可重复的技术，可在B恶性肿瘤患者进行自体骨髓移植（ABMT）前使用多种单克隆抗体混合物清除肿瘤细胞。然而，只有一项使用自体骨髓净化或未净化的随机试验才能阐明该方法的有效临床价值。
