Stoppa A M, Hirn J, Blaise D, Delaage M, Novakovitch G, Viens P, Capiello M A, Mannoni P, Mawas C, Maraninchi D
Marrow Transplant Unit, Clinique des Maladies du Sang, Institut Paoli-Calmettes, Marseille, France.
Bone Marrow Transplant. 1990 Nov;6(5):301-7.
We report here 16 autologous bone marrow transplantations (ABMT) for poor prognosis B or pre-B malignancies (16 acute lymphoblastic leukemias (ALL), three Burkitt lymphomas, one multiple myeloma) in 11 adults and five children where in vitro purging was accomplished by means of floating immunobeads. This method was developed to avoid non-specific killing by complement or toxin or batch-to-batch variability and provides a 3 log reduction of tumor in a model of B lymphoid malignancies. Low density bone marrow mononuclear cells were incubated for 30 min at 4 degrees C with anti CD10 (ALB2 Immunotech) and/or anti CD19 (Bg4) monoclonal antibodies (MoAb) and then mixed with low density polypropylene beads precoated with a rat antimouse MoAb. After 1 h at 4 degrees C the beads with target cells were decanted; the depleted marrow was collected through a microfilter and cryoperserved. After immunodepletion the recovery of nucleated cells was 75% with a median of 0.75 x 10(8) cells/kg (range 0.3-3.6) and the recovery of hematopoietic progenitors was 83% with a median of 2.9 x 10(4) CFU-GM/kg. The conditioning regimen consisted of busulfan 16 mg/kg and melphalan 140 mg/m2 for three patients, fractionated total body irradiation (TBI) following melphalan 140 mg/m2 for nine patients, TBI and cyclophosphamide 120 mg/m2 for two patients and TBI associated with melphalan and cyclophosphamide for two patients.(ABSTRACT TRUNCATED AT 250 WORDS)
我们在此报告16例自体骨髓移植（ABMT），用于治疗预后不良的B系或前B系恶性肿瘤（16例急性淋巴细胞白血病（ALL）、3例伯基特淋巴瘤、1例多发性骨髓瘤），患者为11名成人和5名儿童，其中体外净化通过浮动免疫磁珠完成。开发该方法是为了避免补体或毒素的非特异性杀伤或批次间差异，并在B淋巴细胞恶性肿瘤模型中使肿瘤减少3个对数级。低密度骨髓单个核细胞在4℃下与抗CD10（ALB2 Immunotech）和/或抗CD19（Bg4）单克隆抗体（MoAb）孵育30分钟，然后与预包被大鼠抗小鼠MoAb的低密度聚丙烯磁珠混合。4℃孵育1小时后，倒出带有靶细胞的磁珠；通过微滤器收集耗尽的骨髓并冷冻保存。免疫清除后，有核细胞回收率为75%，中位数为0.75×10⁸细胞/kg（范围0.3 - 3.6），造血祖细胞回收率为83%，中位数为2.9×10⁴CFU - GM/kg。预处理方案包括：3例患者采用白消安16mg/kg和美法仑140mg/m²；9例患者在美法仑140mg/m²后进行分次全身照射（TBI）；2例患者采用TBI和环磷酰胺120mg/m²；2例患者采用TBI联合美法仑和环磷酰胺。（摘要截于250字）
