Gravendyck M, Tritt S, Spenkoch-Piper H, Kaleta E F
Institut fur Geflugelkrankheiten, Justus-Liebig-Universitat, Giessen, Germany.
Avian Pathol. 1996 Jun;25(2):345-57. doi: 10.1080/03079459608419145.
A total number of 28 viruses were isolated from different diseased or dead psittacine birds on various occasions. All of them were end-point purified and identified as herpesviruses (HV) on the basis of chloroform sensitivity, inhibition of multiplication in cell cultures by 5 iodo 2'-deoxyuridine (IUDR), lack of haemagglutination and morphology by electron microscopy. Antisera were produced in rabbits with each of the viruses. Herpesviruses from other birds (duck plague HV, stork HV, crane HV) were used for comparison. All virus/antiserum combinations were tested for homologous and heterologous neutralization in chick embryo fibroblast (CEF) cultures. The r1 and r2, as well as R-values were calculated according to the formula of Archetti & Horsfall (1950). These values formed the basis of the production of minimal trees and dendrograms. None of the non-psittacine viruses were neutralized with antisera produced against any of the psittacine herpesviruses and vice versa. Within the psittacine viruses three distinguishable subtypes were established. It is proposed to use as representative HV for subtype 1, 2 and 3 the strains KS 144/79, 6840/87 and 3115/88, respectively. Isolates 1483/92 and 8326/87 were distinguishable from each other and all other isolates; they are candidates for possible subtypes 4 and 5.
在不同时间从患病或死亡的鹦鹉中总共分离出28种病毒。所有病毒均经终点纯化，并根据氯仿敏感性、5-碘-2'-脱氧尿苷（IUDR）对细胞培养中病毒增殖的抑制作用、缺乏血凝性以及电子显微镜下的形态学特征鉴定为疱疹病毒（HV）。用每种病毒对家兔进行免疫以制备抗血清。使用来自其他鸟类的疱疹病毒（鸭瘟HV、鹳HV、鹤HV）进行比较。在鸡胚成纤维细胞（CEF）培养物中对所有病毒/抗血清组合进行同源和异源中和试验。根据Archetti和Horsfall（1950年）的公式计算r1和r2以及R值。这些值构成了生成最小树和系统树状图的基础。用针对任何鹦鹉疱疹病毒产生的抗血清均不能中和非鹦鹉病毒，反之亦然。在鹦鹉病毒中确定了三种可区分的亚型。建议分别将KS 144/79、6840/87和3115/88菌株用作1型、2型和3型的代表性HV。分离株1483/92和8326/87彼此以及与所有其他分离株都可区分；它们可能是4型和5型的候选亚型。
