Department of Biochemistry and Microbiology, Universiti Pertanian Malaysia, Serdang, Selangor, Malaysia.
Avian Pathol. 1996 Dec;25(4):837-44. doi: 10.1080/03079459608419185.
The nucleotide sequence of the haemagglutinin-neuraminidase (HN) glycoprotein gene of Newcastle disease virus (NDV) variant strain V4(UPM) was determined by direct genomic RNA sequencing and confirmed by cycle sequencing. The gene comprises 1996 nucleotides encoding a 615 amino acid protein of size 67.4 kDa. The nucleotide and amino acid sequences of this strain were compared with those of the parent strain V4(QUE). There are 16 nucleotide substitutions on V4(UPM), eight of which are silent mutations and another eliminated a potential Asn-linked glycosylation site in V4(UPM). In addition, an Arg (403) residue was shown to be absent in the variant strain. This deletion is thought to be significant because of its location in a highly conserved region of the HN protein.
新城疫病毒（NDV）变异株 V4（UPM）血凝素-神经氨酸酶（HN）糖蛋白基因的核苷酸序列通过直接基因组 RNA 测序确定，并通过循环测序进行了验证。该基因包含 1996 个核苷酸，编码 615 个氨基酸的 67.4 kDa 大小的蛋白质。该毒株的核苷酸和氨基酸序列与亲本毒株 V4（QUE）进行了比较。V4（UPM）有 16 个核苷酸取代，其中 8 个是沉默突变，另一个消除了 V4（UPM）中潜在的天冬酰胺连接糖基化位点。此外，变异株中还缺失了一个精氨酸（403）残基。由于该缺失位于 HN 蛋白高度保守区域，因此被认为具有重要意义。
